西瓜果实网条突变体的转录组分析  

Transcriptome Analysis of the Netted Mutant in Watermelon

作　　者：王志强[1] 杜慧莹[1] 李程[1] 郭松[1] 田梅[1] 杨万邦 于蓉[1] WANG Zhiqiang;DU Huiying;LI Cheng;GUO Song;TIAN Mei;YANG Wanbang;YU Rong(Institute of Horticulture,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002)

机构地区：[1]宁夏农林科学院园艺研究所,银川750002

出　　处：《植物遗传资源学报》2024年第8期1385-1395,共11页Journal of Plant Genetic Resources

基　　金：农业高质量发展和生态保护科技创新示范项目(NGSB-2021-7-02)。

摘　　要：以条纹自交系西瓜(TD)和网条突变体西瓜(WT)为材料,通过分离群体的条纹性状比例探索西瓜的条性状遗传基础;以转录组测序及表达差异分析为基础,探索网条突变体西瓜在不同发育阶段的表达谱变化,筛选出条纹自交系与网条突变体西瓜之间表达差异显著的基因57个,主要富集于光合作用天线蛋白通路、生物碱生物合成通路、氨基酸代谢通路等7种代谢途径;通过加权基因共表达网络对核心基因的进一步分析,初步挖掘出部分网条突变体西瓜中有关生物胁迫抗性的基因变化情况,进一步筛选确定西瓜深绿条纹基因(ClGS,watermelon dark-green stripe)、类半胱氨酸蛋白酶抑制剂5编码基因、UDP-葡萄糖基转移酶编码基因、类长春碱合成酶编码基因、叶绿素a-b结合蛋白编码基因可能是决定西瓜网条果皮性状的核心基因;qRT-PCR验证结果表明,条纹自交系西瓜与网条突变体西瓜在基因表达上存在明显差异。本研究为西瓜不同果皮条纹育种提供了新的种质资源,也为阐明西瓜不同果皮条纹性状形成的分子调控机制提供了一定的理论依据。In order to explore the genetic basis of netted rind in watermelon,segregated population was constructed by hybridization between the inbred line(TD)that with striped rind and the mutant line(WT)that with netted rind.The segregation patterns in F2,BC1P1 and BC1P2 populations showed that the striped rind is controlled by a single dominant gene.Transcriptome sequencing and differential expression analysis were conducted for TD and WT rind at different developmental stages.A total of 57 differentially expressed genes(DEGs)were identified between the two genotypes,primarily enriched in seven metabolic pathways,including photosynthesis,alkaloid biosynthesis,and amino acid metabolism.Further exploration of hub genes through weighted gene co-expression network analysis(WGCNA)revealed those related to biotic stress resistance in the netted mutant.Key genes encoding ClGS,cystatin 5,UDP-glucosyltransferase,vinorine synthase,and chlorophyll a-b binding protein were identified as potential determinants of the netted rind.qRT-PCR was applied to validate partial DEGs.This study provides new germplasm resources for watermelon breeding with various rind patterns,and offers a theoretical basis for elucidating the molecular regulatory mechanisms underlying the formation of the rind patterns in watermelon.

关 键 词：西瓜 果皮条纹 转录组 加权基因共表达网络 突变体 

分 类 号：S651[农业科学—果树学]