土壤修复剂协同昆虫病原线虫对黄瓜根际土壤线虫群落的影响  

Effect of Entomopathogenic Nematode Combining with Soil Amendment on Soil Nematode Community Structure in Rhizosphere of Cucumber

作　　者：符慧娟 张海婷 易军 曾铄程 许秉智 叶建蓉 李其勇[1] 张鸿[3] 李星月[1] FU Huijuan;ZHANG Haiting;YI Jun;ZENG Shuocheng;XU Bingzhi;YE Jianrong;LI Qiyong;ZHANG Hong;LI Xingyue(Institute of Plant Protection,Sichuan Academy of Agricultural Sciences/Key Laboratory of Integrated Crop Pest Management in Southwest China,Ministry of Agriculture and Rural Affairs,Chengdu 610066;Agriculture and Rural Affairs Bureau of Yuexi County,Liangshan,Sichuan 616650;Sichuan Academy of Agricultural Sciences,Chengdu 610066)

机构地区：[1]四川省农业科学院植物保护研究所/农业农村部西南作物有害生物综合治理重点实验室,成都610066 [2]越西县农业农村局,四川凉山616650 [3]四川省农业科学院,成都610066

出　　处：《中国农学通报》2024年第28期134-142,共9页Chinese Agricultural Science Bulletin

基　　金：国际科技交流合作提升行动计划专项“中国-以色列应对成渝极端气候适应的草地贪夜蛾生物防治技术研究”(2024ZSSFGH06);四川省科技计划项目“马铃薯孢囊线虫疫情监测及防控关键技术研究”(2021YFN0009);四川省自然科学基金项目“基于极端气候适应能力的昆虫病原线虫高效防治草地贪夜蛾技术研究”(2022NSFSC0170)。

摘　　要：为探索土壤修复剂(soil amendment,SA)协同昆虫病原线虫(Heterorhabditis beicherriana)对南方结节线虫(Meloidogyne incognita)的抑制作用以及对黄瓜根际土壤生态环境的影响,以‘千秋1号’黄瓜为试验品种,采用室内盆栽试验方法,通过设置6个试验组对黄瓜根际土壤线虫群落结构、生态多样性和功能多样性等方面进行研究。结果显示,试验60 d后施入SA和昆虫病原线虫相比其他处理根结植物类寄生线虫减少,小杆属和中杆属等食细菌类线虫增多;营养类群指数和基础成分指数均有所回升,线虫通路比指数显著升高,土壤有机分解以细菌分解为主;线虫区系仍位于土壤养分较好且以细菌降解为主的A区,其他组则从A区逐渐下降到D区。研究表明,SA协同昆虫病原线虫能有效抑制南方根结线虫,同时可以提高土壤线虫的多样性,有效缓解土壤食物网结构退化的趋势。To investigate the inhibitory effect of soil amendment(SA)in combination with entomopathogenic nematode(Heterorhabditis beicherriana)on Meloidogyne incognita,and its impact on the ecological environment of cucumber rhizosphere soil,we used the cucumber cultivar‘Qianqiu No.1’as the test variety,and set up six different experimental groups to study the community structure of soil nematode,ecological diversity and functional diversity of cucumber rhizosphere soil.The results showed that after 60 days of experiment,the SA and nematode H.beicherriana group contained fewer parasitic nematodes in root nodules,and more Rhabditis and Mesorhabditis nematodes.The trophic diversity and basal index were increased,and the nematode channel ratio was significantly increased,and the soil organic decomposition was mainly bacterial decomposition.The nematode population remained predominantly in area A,characterized by better soil nutrients and bacterial decomposition,whereas in other groups,there was a gradual decrease from area A to area D.The results showed that the SA soil restorer combined with entomopathogenic nematode(H.beicherriana)could effectively inhibit M.incongnita,and at the same time improve the diversity of soil nematodes,effectively alleviate the degradation trend of soil food web.

关 键 词：土壤修复剂 昆虫病原线虫 南方根结线虫 黄瓜 群落结构 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]