基于生物碱指纹图谱和多组分定量分析的吴茱萸质量评价  

Study on quality of Evodiae Fructus based on fingerprint of alkaloid components and multi index quantitative analysis

作　　者：姜军华 陈建兰 刘绪平 许妍 JIANG Jun-hua;CHEN Jian-lan;LIU Xu-ping;XU Yan(NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine,Jiangxi Province Engineering Research Center of Drug and Medical Device Quality,Jiangxi Institute for Drug Control,Nanchang 330029,China;Nanchang County Hospital of TCM,Nanchang 330200,China)

机构地区：[1]江西省药品检验检测研究院,国家药品监督管理局中成药质量评价重点实验室,江西省药品与医疗器械质量工程技术研究中心,江西南昌330029 [2]南昌县中医医院,江西南昌330200

出　　处：《化学工程师》2024年第10期25-29,34,共6页Chemical Engineer

基　　金：江西省中医药标准化研究项目(2021A12)。

摘　　要：目的建立吴茱萸生物碱类成分HPLC指纹图谱,测定其中吴茱萸碱、吴茱萸次碱、吴茱萸新碱3个生物碱的含量,并结合聚类分析对不同产地吴茱萸进行质量评价。方法采用HPLC法,以吴茱萸碱为参照,绘制11批吴茱萸的指纹图谱,并进行相似度评价、聚类分析;采用HPLC测定3个生物碱类成分的含量。结果建立了吴茱萸HPLC指纹图谱,确定了11个共有峰,指认出4个峰,与对照指纹图谱的相似度在0.898~0.996之间,不同产地的吴茱萸所含生物碱类成分种类和数量基本一致,3个生物碱类成分的含量在较大范围内波动较大。11批样品可聚为3类。结论该方法简单、可靠、重复性良好,可有效、全面用于吴茱萸质量的评价。OBJECTIVE To establish fingerprints of Evodiae Fructus by HPLC,determine the content of evodiamine,rutaecarpine,evocarpine three alkaloids,and the quality of Evodiae Fructus from different producing companies was evaluated by cluster analysis.METHODS To draw 11 batches Evodiae Fructus of fingerprints by HPLC with evodiamine as the reference,followed by similarity evaluation and cluster analysis;HPLC was used to determine the content of three alkaloid components.RESULTS The HPLC fingerprint of Fructus Evodiae was established with 11 common peaks identified and 4 peaks identified.The similarity with the control fingerprint ranged from 0.898~0.996.The types and quantities of alkaloids in Evodiae Fructus from different regions were basically the same,and the content of the three alkaloids fluctuated significantly within a large range.11 batches of samples can be grouped into 3 categories.CONCLUSION This method is simple,reliable,and has good repeatability,and can be effectively and comprehensively used for the quality evaluation of Evodiae Fructus.

关 键 词：吴茱萸 HLPC 指纹图谱 生物碱 吴茱萸碱 吴茱萸次碱 吴茱萸新碱 

分 类 号：R284.1[医药卫生—中药学]