羟氨强化硫自养反硝化与厌氧氨氧化协同脱氮效果  

Synergistic denitrification effect of hydroxylamine enhanced sulfur autotrophic denitrification and anaerobic ammonia oxidation

作　　者：张雨彤 郭进周 杨永霞 斯绍雄 任召言 刘力嘉 孙志超 刘春爽[3] 刘芳[3] ZHANG Yutong;GUO Jinzhou;YANG Yongxia;SI Shaoxiong;REN Zhaoyan;LIU Lijia;SUN Zhichao;LIU Chunshuang;LIU Fang(Research Institute of Experiment and Detection,Xinjiang Oilfield Company,Karamay 834000,China;Oil and Gas Field Environmental Protection and Energy Conservation Engineering Research Center of Xinjiang Uygur Autonomous Region,Karamay 834000,China;College of Chemical Engineering in China University of Petroleum(East China),Qingdao 266580,China)

机构地区：[1]中国石油新疆油田实验检测研究院,新疆克拉玛依834000 [2]新疆维吾尔自治区油气田环保节能工程研究中心,新疆克拉玛依834000 [3]中国石油大学(华东)化学化工学院,山东青岛266580

出　　处：《中国石油大学学报（自然科学版）》2024年第5期218-224,共7页Journal of China University of Petroleum(Edition of Natural Science)

基　　金：国家自然科学基金项目(21307160);山东省自然科学基金项目(ZR2019MEE038);新疆油田科技攻关项目(2022-C004)。

摘　　要：针对硫化物基质对硫自养反硝化与厌氧氨氧化协同脱氮体系的抑制问题,采用UASB反应器,通过逐渐增加进水羟胺质量浓度实现耦合体系高效脱氮。结果表明:在水力停留时间(t HRT)为4 h,进水NH^(+)_(4)-N和NO^(-)_(3)-N质量浓度分别为60和125 mg/L条件下,实现总氮去除负荷达1.05 kg/(m^(3)·d),其中厌氧氨氧化贡献率达76%~95%,是未投加羟胺时的1.55倍;羟胺的投加能够促进耦合系统污泥heme c合成和EPS(胞外聚合物)的分泌,NH_(2)OH与S^(2-)质量比超过0.024时有助于提高SAA(最大比厌氧氨氧化活性)值;厌氧氨氧化菌Candidatus_Kuenenia丰度和活性在羟胺存在时明显提高,从而与硫自养反硝化菌Thiobacillus、Thiothrix、Sulfurimonas、Sulfurovum和Sulfuritalea协同作用实现高效脱氮。In view of the inhibitory effect of sulfide substrate on the performance of the coupling autotrophic sulfur denitrification and anammox system,hydroxylamine was added gradually into the system to achieve efficient nitrogen removal using UASB reactor.The results show that the total nitrogen removal load reaches as high as 1.05 kg/(m^(3)·d)at HRT of 4 h with the influent NH^(+)_(4)-N and NO^(-)_(3)-N concentration of 60 mg/L and 125 mg/L,respectively.The contribution of anammox for TN removal is among 76%~95%,which is 1.55 times that without NH_(2)OH.The addition of hydroxylamine promotes heme c production and EPS secretion,and the ratio of NH_(2)OH to S^(2-)higher than 0.024 is helpful to the enhancement of SAA.The abundance and activity of Candidatus_Kuenenia is significantly increased in the presence of hydroxylamine,so as to achieve efficient nitrogen removal by cooperating with sulfur autotrophic denitrification bacteria Thiobacillus,Thiothrix,Sulfurimonas,Sulfurovum and Sulfuritalea.

关 键 词：羟胺 硫自养反硝化 厌氧氨氧化 脱氮 群落结构 

分 类 号：X703[环境科学与工程—环境工程]