基于红外孔板嗅觉测试仪在小鼠嗅觉功能研究中的应用  

Application of an infrared-based odorized hole-board test instrument in the study of olfactory function in mice

作　　者：刘彤彤 李婧 董朝玉 王灿 解俊樊 侯一平[1] 邵玉峰[1] Liu Tongtong;Li Jing;Dong Chaoyu;Wang Can;Xie Junfan;Hou Yiping;Shao Yufeng(School of Basic Medical Sciences,Lanzhou University,Lanzhou 730000,China;School of Basic Medicine,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;Department of Gynecology,The Third People's Hospital of Yunnan Province,Kunming 650011,China;School of Medical Imaging,Shandong Second Medical University,Weifang 261053,Shandong,China)

机构地区：[1]兰州大学基础医学院,甘肃兰州730000 [2]甘肃中医药大学基础医学院,甘肃兰州730000 [3]云南省第三人民医院妇科,云南昆明650011 [4]山东第二医科大学医学影像学院,山东潍坊261053

出　　处：《兰州大学学报（医学版）》2024年第9期6-13,34,共9页Journal of Lanzhou University（Medical Sciences）

基　　金：国家自然科学基金资助项目(31872770,31500853,81771426,82001396)。

摘　　要：目的利用灵敏的红外传感器取代电容传感器并用于孔板嗅觉测试仪,建立小鼠嗅觉分辨、识别、空间记忆、偏好实验方法,以检验孔板嗅觉测试仪检测小鼠嗅觉功能的稳定性。方法分别采用习惯/去习惯行为测试、嗅觉空间记忆测试、丙酸嗅觉阈值测试和嗅觉偏好检测正常C57 BL/6J小鼠的嗅觉功能。结果C57 BL/6J小鼠具有嗅觉辨别、嗅觉线索关联的空间记忆能力,对丙酸的嗅觉阈值为10^(-3)kPa,对香叶醇的偏好度最高,对左旋香芹酮的偏好度最低。结论本研究使用的孔板嗅觉测试仪能够满足小鼠嗅觉的辨别、识别、学习记忆以及偏好性等嗅觉行为学实验需求。Objective To establish experimental methods of olfactory discrimination,recognition,spatial memory and preference in mice by using sensitive infrared sensors instead of capacitive sensors and using them in the odorized hole-board test instrument in order to test the stability of the olfactory functions of mice studied by the instrument.Methods Habituation/dishabituation test,olfactory spatial memory test,propionic acid olfactory threshold test and olfactory preference test were used to evaluate the olfactory function of normal C57 BL/6J mice.Results C57 BL/6J mice were capable of olfactory discrimination and spatial memory of olfactory cue association.The olfactory threshold for propionic acid was 10^(-3) kPa,and the preference for geraniol the highest,while the preference for L-carvone the lowest.Conclusion The infrared-based odorized hole-board test instrument used in this study can satisfy the requirements of olfactory behavior experiments such as discrimination,recognition,learning and memory,and preference of mice.

关 键 词：嗅觉 嗅觉功能 嗅觉行为学 嗅觉测试仪 红外传感器 小鼠 

分 类 号：R338[医药卫生—人体生理学]