濒危植物百山祖冷杉种子活力与种子物理指标相关性分析  

Correlation analysis between physical characteristics and seed vigor of the endangered plant Abies beshanzuensis

作　　者：吕洪飞 楼晨坤[2] 兰荣光 熊艳云 杨云芳 于明坚 LYU Hongfei;LOU Chenkun;LAN Rongguang;XIONG Yanyun;YANG Yunfang;YU Mingjian(School of Civil Engineering and Architecture,Zhejiang Guangxia Construction Vocational and Technical University,Dongyang,Zhejiang 322100,China;Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation,College of Life Science and Medicine,Zhejiang Sci-Tech University,Hangzhou 310028,China;Qingyuan Conservation Center of Qianjiangyuan-Baishanzu National Park,Qingyuan,Zhejiang 323800,China;College of Life Science,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江广厦建设职业技术大学建筑工程学院,浙江东阳322100 [2]浙江理工大学生命科学与医药学院,浙江省植物次生代谢调控重点实验室,杭州310028 [3]钱江源-百山祖国家公园庆元保护中心,浙江庆元323800 [4]浙江大学生命科学学院,杭州310058

出　　处：《西北植物学报》2024年第10期1549-1556,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：百山祖国家公园科研项目百山祖冷杉专项(2021ZDZX04);百山祖国家公园科研项目(2023JBGS02)。

摘　　要：【目的】探究百山祖冷杉种子的形态特征和物理指标与种子活力之间的关系,为研究该濒危物种种群更新、保护及解释其濒危机制和其种子生物学提供理论依据。【方法】以极度濒危植物百山祖冷杉为材料,测定百山祖冷杉种子长度、宽度、厚度、单粒重、RGB值等特征常数,用TTC染色法测定其种子活力,并建立数学模型。【结果】百山祖冷杉有活力和无活力种子的R、B值和单粒重存在显著差异,单因素线性回归分析表明,百山祖冷杉种子活力与种子单粒重显著相关,R2为0.839;双因素线性回归分析表明,种子活力与种子单粒重和宽度显著相关,R2为0.895;多因素线性回归分析,R2为0.978;但其有活力种子和无活力种子长度、宽度、厚度和G值不存在显著差异。【结论】用百山祖冷杉种子的R、B值和单粒重等指标可筛选具有较高种子活力的种子,用多因素线性回归分析可更精确分析其种子活力。[Objective]This study aims to explore the relationship between seed morphological and physical characteristics and seed vigor of Abies beshanzuensis,in order to provide a theoretical basis for further population renewal and protection of this endangered species,explaining its endangered mechanism,and studying its seed biology.[Methods]Using the endangered plant A.beshanzuensis as material,characteristic constants of the length,width,thickness,single grain weight,and RGB,etc.of the seeds of A.beshan zuensis were measured.Seed vigor was determined using TTC staining method,and their mathematical models were established.[Results]Significant differences were observed in R,B values,and single seed weight between vigorous and non-viable seeds of A.beshanzuensis.Single factor linear regression analysis showed that the correlation between seed vigor and single seed weight of A.beshanzuensis was the most significant,with R2=0.839.Two factor linear regression analysis showed that seed vigor had the most significant correlation with single seed weight and width,with R2=0.895.For multivariate linear regres-sion analysis,R2=0.978.However,no significant differences were observed in length,width,thick-ness,and G value between vigorous and non-viable seeds.[Conclusion]By using indicators such as R,B value,and single grain weight of A.beshanzuensis seeds,seeds with higher seed vigor can be screened.Multiple factor linear regression analysis predicts more accurately the seed vigor.

关 键 词：百山祖冷杉 TTC染色 形态特征 物理特征 单粒重 种子活力 数学模型 

分 类 号：Q947[生物学—植物学] S791.14[农业科学—林木遗传育种]