茶菊茎尖分生组织高效脱毒再生体系优化  

Optimization of efficient virus-free regeneration system from shoot apical meristem of tea chrysanthemum

作　　者：史子尹 张海楠 李浩 吕晋慧[1] SHI Ziyin;ZHANG Hainan;LI Hao;LYU Jinhui(College of Forestry,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China)

机构地区：[1]山西农业大学林学院,山西晋中030801

出　　处：《西北植物学报》2024年第10期1557-1566,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：山西省现代农业产业技术体系建设育种岗位专家项目(2021-11)。

摘　　要：【目的】筛选适宜热处理时间和脱毒剂,优化茶菊茎尖分生组织高效脱毒再生体系,解决脱毒苗获得率低的问题。【方法】以6个茶菊品种无菌苗为材料,采用昼/夜40℃/32℃变温、化学脱毒剂及其组合预处理无菌苗,比较茎尖分生组织的出愈率、再生频率和平均再生芽数及不同方法脱毒效果。【结果】茎尖分生组织再生频率在不同品种茶菊间差异显著,并以‘婺源皇菊’最高(54.77%);茎尖分生组织再生在不同昼夜变温热处理时长间差异显著,以‘杭白菊’、‘七月雪’再生效果最好。‘婺源皇菊’茎尖分生组织再生在脱毒剂水杨酸、利巴韦林、脱落酸处理下均受到抑制,在水杨酸、利巴韦林结合变温热处理下得到促进,在高浓度脱落酸结合热处理下受到严重抑制,且10μmol/L水杨酸结合热处理的促进效果最好。在适宜热处理、化学脱毒剂结合热处理可获得茶菊完全脱毒苗。【结论】10μmol/L水杨酸浓度结合40℃/32℃昼/夜变温预处理40d能显著提高茎尖分生组织再生频率,获得脱毒苗。[Objective]In order to optimize the efficient virus elimination and regeneration system from shoot apical meristem of tea chrysanthemum,suitable heat treatment duration and detoxification agents,this study aims to improve the problem of low virus-free seedlings obtaining ratio.[Methods]Six tea chrysanthemum varieties were used as materials.The axenic seedlings were pre-treated with alternating heat temperature treatment of 40℃/32℃day/night,detoxification agents such as salicylic acid,ribavirin,and abscisic acid,as well as a combination of detoxification agents and heat treatment.The rate of callus induction,regeneration frequency,and average number of regenerated shoots from inoculated shoot apical meristems were compared among different treatment methods,the detoxification efficacy of various treatments were evaluated.[Results]There was a significant difference in the regeneration frequency of shoot apical meristems among different varieties of tea chrysanthemum,with‘Wuyuan Huangju’having the highest regeneration frequency of 54.77%.Under different heat treatment days,the difference in re-generation of shoot apical meristems in tea chrysanthemum reached a significant level,with‘Hangbaiju’and‘Qiyuexue’showing the best regeneration after 40℃/32℃heat treatment.Salicylic acid,ribavirin,and abscisic acid treatments inhibited the regeneration of shoot apical meristems.Salicylic acid and ribavi-rin treatments combined with heat treatment promoted the regeneration of shoot apical meristems,while high concentration abscisic acid combined with heat treatment severely inhibited the regeneration of shoot apical meristems.Treatment with 10μmol/L salicylic acid combined with heat treatment showed the best regeneration.Virus-free seedlings were obtained through suitable heat treatment combined with detoxifica-tion agents.[Conclusion]The combination of 10μmol/L salicylic acid with 40℃/32℃day/night pre-treatment for 40 days significantly enhances the regeneration frequency of shoot apical meristems,and

关 键 词：茶菊 茎尖分生组织 热处理 化学脱毒剂 再生体系 脱毒 

分 类 号：S682.11[农业科学—观赏园艺]