积雪草主要三萜类化合物测定方法的优化研究  

Optimization of methods for determining major triterpenoid compounds in Centella asiatica(L.)Urban

作　　者：杨若朦 田军[1] 李蕴哲 YANG Ruomeng;TIAN Jun;LI Yunzhe(Xi'an University,Xi'an 710065,China;Xi'an Jiaotong University,Xi'an 710061,China)

机构地区：[1]西安文理学院,西安710065 [2]西安交通大学,西安710061

出　　处：《西北植物学报》2024年第10期1639-1645,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：陕西省重点研发计划项目(2019SF-277)。

摘　　要：【目的】建立以环糊精为流动相添加剂的高效液相色谱法测定积雪草药材中三萜类化合物积雪草苷、羟基积雪草苷和积雪草苷B的含量,为控制积雪草的质量提供依据。【方法】采用分子设计方法优选流动相添加剂-环糊精种类,效应面法分析环糊精浓度、流动相中乙腈的比例、流动相pH对分离的影响,优化色谱条件,测定积雪草中积雪草苷、羟基积雪草苷和积雪草苷B含量。【结果】优化后HPLC色谱测定方法以γ-环糊精为流动相添加剂,固定相为CAPCELLPAKC18柱(150mm×4.6mm,5μm),流动相为乙腈-3mmol/L环糊精溶液(含0.10%磷酸)=20∶80(V/V),流速1.0mL/min,柱温25℃,检测波长205nm;羟基积雪草苷和积雪草苷B分离度为4.32;积雪草苷为200~2000ng,积雪草苷B为120~1200ng,羟基积雪草苷为200~2000ng内线性关系良好,方法精密度、重现性、稳定性和回收率良好。【结论】新HPLC色谱测定方法简便、准确、可靠,且经济环保,可用于测定积雪草药材中主要三萜类化合物。[Objective]In order to provide a basis for quality control of Centella asiatica(L.)Urban,a high-performance liquid chromatography method was established using cyclodextrin as a mobile phase additive for the determination of asiaticoside,madecassoside,and asiaticoside B.[Methods]Molecular design was used to optimize the type of the mobile phase additive-cyclodextrin.The response surface method was used to analyze the effects of cyclodextrin concentration,the proportion of acetonitrile in the mobile phase,and pH of the mobile phase on separation,as well as to optimize the chromatographic condition.The optimized chromatographic condition was used to determine the content of asiaticoside,madecassoside,and asiaticoside B in C.asiatica.[Results]In the optimization of determination method,γ-cyclodextrin was used as a mobile phase additive.The separation of asiaticoside,asiaticoside B,and madecassoside was carried out on CAPCELL PAK C18(150 mm×4.6 mm,5μm)with acetonitrile-3 mmol/Lγ-cyclodextrin solution(contained 0.10%phosphoric acid)(20∶80,V/V).The detecting wavelength was 205 nm.The flow rate was set at 1.0 mL/min and the column temperature was 25℃.The resolution of madecassoside and asiaticoside B was 4.32.Linearity was good in the range of 200-2000 ng for asiaticoside,120-1200 ng for asiaticoside B,and 200-1200 ng for madecassoside.The precision,reproducibility,stability,and recovery rate of method were good.[Conclusion]The new method is simple,accurate,reliable,and envi-ronmentally friendly,and can be used as a quality control of major triterpenoid compounds in C.asiatica(L.)Urban.

关 键 词：积雪草 三萜类化合物 环糊精流动相添加法 分子设计 效应面法 

分 类 号：Q946[生物学—植物学] S567.239[农业科学—中草药栽培]