旱柳失水枝条中ABA介导的代谢组响应分析  

Analysis of ABA-mediated metabolome response in dehydrated branches of Salix matsudana

作　　者：胡耀芳 李奥 殷佳慧 王元成 刘俊祥[1] 孙振元[1] HU Yaofang;LI Ao;YIN Jiahui;WANG Yuancheng;LIU Junxiang;SUN Zhenyuan(Key Laboratory of Tree Breeding and Cultivation of the National Forestry and Grassland Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China)

机构地区：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091

出　　处：《西北植物学报》2024年第11期1735-1742,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(32071729)。

摘　　要：【目的】ABA是植物干旱胁迫的关键激素,通过代谢组学分析揭示旱柳离体枝条ABA介导的失水响应差异代谢物及其相关通路,为旱柳干旱胁迫响应提供研究基础和理论依据。【方法】以旱柳离体枝条为材料,探究ABA合成抑制剂钨酸钠溶液浸泡后旱柳失水至不同水势枝条木质部代谢组的差异响应。【结果】失水至P50(-1.9 MPa)旱柳枝条代谢组与未失水枝条相比,内源ABA含量显著增加了425.032%,D-果糖等可溶性糖含量与L-谷氨酸等氨基酸含量显著增加。ABA抑制剂处理后内源ABA含量显著降低了79.253%,D-1,5-脱水果糖等可溶性糖含量与苯丙氨酸等氨基酸含量显著降低。植物激素信号转导、类胡萝卜素生物合成和淀粉与蔗糖代谢途径等是旱柳枝条失水响应的关键代谢通路。【结论】旱柳枝条失水后ABA含量显著增加,介导多糖水解过程等降低了细胞渗透势以应对干旱胁迫。[Objective]ABA is a key hormone for drought stress in plants.Differential metabolites and their associated pathways of ABA-mediated dehydration response in the isolated branches of Salix matsudana were investigated by metabolomics analysis,which provided the basis for the response to drought stress in S.matsudana.[Methods]The authors investigated the differential response of the xylem metabolomes of the S.matsudana branches dehydrated to different water potentials after soaked in the sodium tungstate solution,an inhibitor of ABA synthesis,using the isolated branches of S.matsudana as materials.[Results]Compared with the deionized water-soaked non-dehydrated branches,in the dehydrated to the P50 water potential branches the endogenous ABA content was significantly increased by 425.032%,and content of the soluble sugars such as D-fructose was significantly increased along with the content of amino acids such as L-glutamic acid.When ABA synthesis inhibitor-soaked branches was com pared to deionized water-soaked branches,the content of endogenous ABA was significantly decreased by 79.253%,and the content of the soluble sugars such as D-1,5-defructose and amino acids such as phenyl-alanine were significantly reduced after soaked in the inhibitor of ABA synthesis.Plant hormone signal transduction,carotenoid biosynthesis and starch and sucrose metabolism pathways were the key pathways in the dehydrated response of S.matsudana branches.[Conclusion]In the S.matsudana branches after dehydration,the ABA content is increased significantly,mediating the polysaccharide hydrolysis process and other processes to reduce the osmotic potential in response to drought stress.

关 键 词：旱柳 水势 ABA 代谢组 差异代谢物 代谢通路 

分 类 号：Q945.78[生物学—植物学] S792.12[农业科学—林木遗传育种]