体电子显微成像技术在肝癌细胞超微结构分析中的应用研究  

Application of volume electron microscopy in ultrastructure analysis of hepatocarcinoma cells

作　　者：郭建胜[1] 张兴 GUO Jiansheng;ZHANG Xing(Center of Cryo-Electron Microscopy,Zhejiang University School of Medicine,Hangzhou Zhejiang 310058,China)

机构地区：[1]浙江大学医学院冷冻电镜中心,浙江杭州310058

出　　处：《电子显微学报》2024年第4期479-486,共8页Journal of Chinese Electron Microscopy Society

基　　金：浙江大学实验技术项目资助(No.SYBJS202207).

摘　　要：体电子显微成像技术(volume electron microscopy)可以在更大三维空间中对样品进行纳米分辨率三维结构分析,获取样品内部结构的三维模型和各结构之间的位置关系、体积比例等信息,更加全面地反映样品的超微结构与功能的关系。本文利用基于聚焦离子束扫描电镜的体电子显微成像技术对人源肝癌细胞的三维超微结构进行分析,获得了多种细胞器包括细胞核、线粒体、内质网和高尔基体等的高分辨率三维结构模型。Volume electron microscopy(vEM)enables the analysis of three⁃dimensional(3D)structures at nanometer resolution within a large 3D space.This technique allows researchers to construct 3D models of the internal structure of samples,providing a comprehensive view of their ultrastructure.Additionally,vEM offers valuable insights into the positional relationships and volume ratios of different structures within the sample.In this study,vEM based on focused ion beam scanning(FIB)was employed to analyze the 3D ultrastructure of human hepatocarcinoma cells.High⁃resolution 3D structures of various organelles,including the nucleus,mitochondria,endoplasmic reticulum and Golgi apparatus,were successfully obtained.

关 键 词：聚焦离子束扫描电镜 体电子显微成像技术 人源肝癌细胞 三维重构 超微结构 

分 类 号：Q248[生物学—细胞生物学] Q336