非人灵长类多能干细胞体外培养和诱导分化的研究进展  

Research Progress on in vitro Culture and Induced Differentiation of Non-human Primate Pluripotent Stem Cells

作　　者：沈可成 朱家桥[1,2] 刘宗平 SHEN Kecheng;ZHU Jiaqiao;LIU Zongping(College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou 225009,China)

机构地区：[1]扬州大学兽医学院,扬州225009 [2]江苏高校动物重要疫病与人畜共患病防控协同创新中心,扬州225009

出　　处：《畜牧兽医学报》2024年第10期4278-4289,共12页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点研发计划(2022YFF0710901);江苏高校优势学科建设工程项目(PAPD)。

摘　　要：干细胞在疾病发生、细胞治疗、药物筛选和临床应用等方面具有巨大价值。自日本科学家Shinya Yamanaka发现诱导多能干细胞以来,相关的研究立即成为研究热点并持续至今。小鼠干细胞的研究为其它动物的相关研究开辟了道路,但在药物筛选和疾病治疗等方面有很大局限性。非人灵长类与人类具有更高的基因同源性,在生理、病理、生殖、药理、神经等多方面与人类高度相似,是研究人类疾病和开发人用药物的理想动物模型。非人灵长类研究也是新药在进入临床实验前必须的一个环节。非人灵长类干细胞的研究在疾病的机理研究和药物开发中越发重要,同时避免了伦理问题。本文从体外培养系统的优化和诱导分化的方法两个方面对非人灵长类多能干细胞的相关研究进行了综述,以期为非人灵长类干细胞进一步深入研究提供参考。Stem cells have great value in disease occurrence,cell therapy,drug screening,and clinical applications.Since Shinya Yamanaka,a Japanese scientist,discovered induced pluripotent stem cells(iPSCs),the relevant research has become a research hotspot and up to the present.The study of mouse stem cells has opened up a path for the related research in other animals,but there are significant limitations in drug screening and disease treatment.Non-human primates have higher genetic homology with humans and are highly similar to humans in physiology,pathology,reproduction,pharmacology,nerves,and other aspects.They are ideal animal models for studying human diseases and developing drugs for human.Research on non-human primates is also a necessary step for a new drugs before entering clinical trials.The study of nonhuman primate stem cells is becoming important in the mechanism of diseases and drug development,while avoiding ethical issues.This article reviews the related research on non-human primate pluripotent stem cells including optimization of in vitro culture systems and methods of in ducing differentiation,aiming to provide reference for further in-depth research on non-human primate stem cells.

关 键 词：非人灵长类 多能干细胞 体外培养 诱导分化 

分 类 号：Q254[生物学—细胞生物学]