丝胶蛋白对承德无角黑山羊精液冷冻保存的影响  

Effect of Sericin on Cryopreservation of Semen of Chengde Hornless Black Goat

作　　者：张文涛 于洋 齐雅天 赵炜 张洪军 陶晨雨 夏威 李俊杰[1,4] ZHANG Wentao;YU Yang;QI Yatian;ZHAO Wei;ZHANG Hongjun;TAO Chenyu;XIA Wei;LI Junjie(College of Animal Science and Technology,Hebei Agricultural University,Baoding 071000,China;Agriculture and Rural Bureau of Fengning Manchu Autonomous County,Fengning 068350,China;Agriculture and Rural Bureau of Weichang Manchu and Mongolian Autonomous County,Weichang 068450,China;Hebei Technology Innovation Center of Cattle and Sheep Embryo,Baoding 071000,China)

机构地区：[1]河北农业大学动物科技学院,保定071000 [2]丰宁满族自治县农业农村局,丰宁068350 [3]围场满族蒙古族自治县农业农村局,围场068450 [4]河北省牛羊胚胎技术创新中心,保定071000

出　　处：《畜牧兽医学报》2024年第10期4466-4474,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：“十四五”国家重点研发计划(2021YFD1200401);河北省科技计划资助项目(17226613D)。

摘　　要：旨在探究丝胶蛋白对承德无角黑山羊精液冷冻保存效果的影响。本试验采用假阴道法采集3只健康状态良好的承德无角黑山羊精液,随机分为6组,分别为对照组和在OptiXcell 2稀释液中添加不同浓度的(0.2%、0.4%、0.6%、0.8%、1%)丝胶蛋白试验组,每组设置5个重复,精液稀释完后进行细管分装、平衡、冷冻;精液冷冻-解冻后检测精子活力、顶体完整率、质膜完整率、总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化歧化酶(SOD)、过氧化氢酶(CAT)活性、丙二醛(MDA)含量、线粒体膜电位、ATP含量等指标,并进行数据统计。结果显示,承德无角黑山羊精液冷冻稀释液中添加不同浓度丝胶蛋白均能够提高冻融后精子质量。其中,添加浓度为0.6%丝胶蛋白与其他各浓度相比,可显著提高精子冷冻-解冻后的精子活力、顶体完整率、质膜完整率以及线粒体膜电位和ATP含量,并且使抗氧化酶T-AOC、GSH-Px、SOD、CAT活性分别提高至(13.61±0.39)mmol·g^(-1)、(26.52±1.30)U·mg^(-1)、(165.45±11.10)U·mg^(-1)、(13.76±0.30)U·mg^(-1),降低MDA值至(3.15±0.20)μmol·mg^(-1)。综上,在冷冻稀释液中添加不同浓度的丝胶蛋白均可提高承德无角黑山羊冷冻精液品质,且最适添加浓度为0.6%。The aim of this study was to explore the effect of sericin on cryopreservation of semen of Chengde hornless black goat.Semen samples of 3 healthy Chengde hornless black goats were collected by the sham vaginal method and randomly divided into 6 groups:control group and experimental groups with different sericin concentrations(0.2%,0.4%,0.6%,0.8%,1%)added to the OptiXcell 2 dilution.Each group was set up with 5 replicates.Sperm motility,acrosome integrity rate,plasma membrane integrity rate,total antioxidant capacity(T-AOC),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),catalase(CAT)activity,malondialdehyde(MDA)content,mitochondrial membrane potential(MMP)and ATP content were detected af-ter freezing and thawing.The results showed that the addition of different concentrations of seri-cin to the semen frozen dilution of Chengde hornless black goat could improve the quality of sperm af-ter freezing and thawing.Compared with other concentrations,the addition of 0.6%sericin significantly increased sperm motility,acrosome integrity rate,plasma membrane integrity rate,mitochondrial mem-brane potential and ATP content after sperm freezing and thawing,and increased the activities of antioxi-dant enzymes T-AOC,GSH-Px,SOD and CAT to(13.61±0.39)mmol·g^(-1),(26.52±1.30)U·mg^(-1),(165.45±11.10)U·mg^(-1)and(13.76±0.30)U·mg^(-1),respectively.Meanwhile,the MDA value was reduced to(3.15±0.20)μmol·mg^(-1).In summary,sericin at different concen-trations can improve the quality of frozen semen of Chengde hornless black goat,and the most suitable concentration is 0.6%.

关 键 词：丝胶蛋白 承德无角黑山羊 抗氧化剂 精液冷冻 

分 类 号：S827.3[农业科学—畜牧学]