新型竹鼠源阿卡斑病毒对山羊的致病性  

Pathogenicity of a Newly Akabane Virus on Goats from Bamboo Rat

作　　者：秦树英 刘金凤 马玲 许力士 杨磊 陈凤莲 韦珊珊 陆晨阳 林俊 韦珏 覃绍敏 吴健敏 QIN Shuying;LIU Jinfeng;MA Ling;XU Lishi;YANG Lei;CHEN Fenglian;WEI Shanshan;LU Chenyang;LIN Jun;WEI Jue;QIN Shaomin;WU Jianmin(Guangxi Key Laboratory of Veterinary Biotechnology,Key Laboratory of China(Guangxi)-ASEAN Cross-border Animal Disease Prevention and Control,Ministryof Agriculture and Rural Affairs of China,Guangxi Veterinary Research Institute,Nanning 530001,China)

机构地区：[1]广西壮族自治区兽医研究所广西兽医生物技术重点实验室,农业农村部(广西)东盟跨境动物疫病防控重点实验室,南宁530001

出　　处：《畜牧兽医学报》2024年第10期4553-4561,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(32160830);广西基本科研业务费(桂科专项20-4);广西基本科研业务费(桂科专项21-7)。

摘　　要：旨在观察竹鼠源阿卡斑病毒(AKAV)对山羊的致病性,本研究以竹鼠源阿卡斑病毒GXLCH16-70株分别经颅内、皮下和静脉感染山羊,观察山羊临床症状、病毒血症、剖检病变、组织病理和病原组织器官分布等指征。结果显示,颅内接种组山羊攻毒第4~9天后,出现间歇性痉挛、抽搐等神经症状;皮下和静脉接种组的山羊于接毒第3~8天出现腹泻、眼角分泌物增多等症状;接种GXLCH16-70的所有山羊于感染第2~10天均出现病毒血症,感染第8天后均产生AKAV中和抗体,且一直持续到第21天。颅内组剖检可见大脑非化脓性脑脊髓炎,肺充血出血、脾边缘有出血点等病理变化。病理组织学检查显示,颅内组呈现为脑膜和皮质血管周围见有多层胶质细胞等炎性细胞浸润,形成“血管套”样结构。荧光定量RT-PCR对脑组织、脊髓等样品进行的病原检测结果显示,颅内组几乎在大脑的所有区域都检测到AKAV-S RNA片段;同时,静脉组和皮下组在部分脑组织和脊髓中也能检测到病原。结果表明,新型竹鼠源AKAV GXLCH16-70株经人工感染可导致山羊全身多器官和组织系统性病变,并且可引起山羊脑脊髓炎。In order to determine the pathogenicity of Akabane virus(AKAV)from bamboo rat in goats,GXLCH16-70 strain was used to infect goats via intracranial(IC),intravenous(IV)and subcutaneous(SC)inoculation.The clinical manifestations,viremia,pathological changes,histopathology,and pathogenic distribution in tissue or organ of goats were observed.The 4th to 9th day post infection(dpi),goats in the IC group showed obvious neurological symptoms such as intermittent spasm,convulsion,while goats in the IV and SC groups showed symptoms as such diarrhea and increased secretions in the eyes at the 3rd to 8th dpi.All goats infected by GXLCH16-70 developed viremia at the 2nd to 10th dpi,and produced AKAV neutralizing antibodies at the 8th dpi.The antibodies continued to exist up to the 21st dpi.Autopsy in the intracranial group showed non-suppurative encephalomyelitis of the brain,pulmonary congestion and hemor-rhage,marginal hemorrhagic spots of the spleen and other pathological changes.Histopathologi-cal examinations on the IC group showed the multiple layers of inflammatory cells infiltrating to the meninges and cortical blood vessels,forming a"vascular sheath"like structure.Pathogen de-tection by fluorescence quantitative RT-PCR(qRT-PCR)showed that AKAV-S RNA fragments were detected in almost all regions of the brain in the IC group,and in some brain tissue and spinal cord in the IV group and SC group.The results showed that AKAV GXLCH16-70 strain from bamboo rat causes systemic lesions of multiple organs and tissues of goats and induce encephalomyelitis.

关 键 词：竹鼠 阿卡斑病毒 山羊 致病性 颅内接种 

分 类 号：S852.659.5[农业科学—基础兽医学]