甲烷马赛球菌DZ1对小鼠血清氧化三甲胺和炎症因子、肝脏抗氧化能力及盲肠微生物区系的影响  

Effects of Methanomassiliicoccus DZ1 on Serum Trimethylamine-N-oxide and Inflammatory Factors,Liver Antioxidant Capacity and Cecum Microbiota in Mice

作　　者：占小秀 刘鹏宇 向小娥[3] 毛胜勇[1,2] 金巍[1,2] ZHAN Xiaoxiu;LIU Pengyu;XIANG Xiao’e;MAO Shengyong;JIN Wei(Ruminant Nutrition and Feed Engineering Technology Research Center,Nanjing Agricultural University,Nanjing 210095,China;Laboratory of Gastrointestinal Microbiology,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;National Experimental Teaching Center of Animal Science,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学反刍动物营养与饲料工程技术研究中心,南京210095 [2]南京农业大学消化道微生物研究室,南京210095 [3]南京农业大学动物科学类国家级实验教学中心,南京210095

出　　处：《畜牧兽医学报》2024年第10期4679-4689,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省自然科学基金(BK20211217);国家自然科学基金(31872381)。

摘　　要：甲烷马赛球菌是哺乳动物消化道中的固有菌群,能够利用三甲胺(TMA)生成甲烷,然而其对宿主的作用机制尚不十分清楚。本文旨在利用小鼠模型研究灌胃甲烷马赛球菌菌株DZ1活菌对小鼠血清氧化三甲胺(TMAO)和炎症因子、肝抗氧化能力及盲肠微生物区系的影响。试验采用14只5周龄雄性C57BL/6J小鼠(体重18.4±1.1 g),随机分为对照组(n=7)和处理组(n=7),单笼饲养。处理组每天灌胃200μL DZ1菌液(1.09×10^(9)个细胞·mL^(-1)),对照组每天灌胃200μL无菌PBS溶液,试验期4周。结果显示,与对照组相比,处理组小鼠日增重和采食量没有显著变化(P>0.05)。处理组小鼠血清TMAO浓度和炎症因子水平显著降低(P<0.05)。处理组小鼠肝中超氧化酶歧化酶(SOD)活性显著升高(P=0.035),肝总抗氧化能力(T-AOC)显著提高(P=0.039)。盲肠细菌16S rRNA基因测序结果显示,处理组和对照组菌群结构没有显著差异(ANOSIM,P=0.161)。处理组中疣微菌门(Verrucomicrobia)的相对丰度有降低的趋势(P=0.064),Lawsonibacter属、瘤胃球菌属(Ruminococcus)和阿克曼菌属(Akkermansia)等的相对丰度显著降低(P<0.05)。综上,灌胃甲烷马赛球菌菌株DZ1未显著影响小鼠盲肠细菌区系结构,但降低了小鼠血清TMAO和炎症因子水平,提高了肝组织总抗氧化能力。Methanomassiliicoccales is an indigenous group of methanogens in the mammalian gut that can use trimethylamine(TMA)to produce methane,but the mechanisms of its action on the host remain unclear.The aim of this study was to investigate the effects of Methanomethylophilus sp.DZ1 on serum trimethylamine-N-oxide(TMAO)and inflammatory factors,liver antioxidant capacity,and cecum microbiota in mice.Fourteen five-week-old male B7/6J mice(body weight 18.4±1.1g)were randomly divided into a control group(n=7)and a treatment group(n=7),single cage feeding.Each mouse in the treatment group was given 200μL DZ1(1.09×10^(9)cells·mL^(-1))daily,and each mouse in the control group was given 200μL sterile PBS solution daily.The experiment lasted for 4 weeks.The results showed that the body weight and feed in-take of mice in the treatment group had no significant changes(P>0.05).Serum TMAO con-centration and inflammatory factor levels of mice in the treatment group were significantly de-creased(P<0.05).The activities of superoxidase dismutase(SOD)in the liver of the treatment group were significantly increased(P=0.035),and the total antioxidant capacity(T-AOC)of the liver was significantly increased(P=0.039).The results of 16S rRNA gene sequencing of cecal bacteria showed no significant difference in bacterial community structure between the treat-ment group and control group(ANOSIM,P=0.161).The relative abundance of Verrucomicro-bia in the treatment group significantly decreased(P=0.064).The relative abundances of Law-sonibacter,Ruminococcus,and Akkermansia were significantly decreased(P<0.05).In summa-ry,the strain DZ1 had no significant effect on the bacterial community structure of the cecum in mice,but decreased the levels of serum TMAO and inflammatory factors,and increased the total antioxidant capacity of the liver.

关 键 词：氧化三甲胺(TMAO) 甲烷马赛球菌 肠道微生物 炎症因子 抗氧化能力 

分 类 号：S852.6[农业科学—基础兽医学]