麦粒灸通过IκBα缓解环磷酰胺小鼠脾脏损伤  

Wheat grain moxibustion relieves cyclophosphamide-induced mouse spleen injury via

作　　者：程艳婷 李洁 侯亚薇 张帮 范旭 丁彦泽 CHENG Yanting;LI Jie;HOU Yawei;ZHANG Bang;FAN Xu;DING Yanze(Second Clinical College,Shanxi University of Chinese Medicine,Jinzhong 030600,China;Department of Traditional Chinese Medicine,Gansu Wuwei Tumour Hospital,Wuwei 733000,China)

机构地区：[1]山西中医药大学第二临床学院,晋中030600 [2]甘肃武威肿瘤医院中医科,733000

出　　处：《免疫学杂志》2024年第4期353-358,共6页Immunological Journal

基　　金：山西省科技厅自然基金面上项目(202303021211177);山西中医药大学校级项目(2023PY-YS-05)。

摘　　要：目的基于NF-κB信号通路分析麦粒灸对环磷酰胺所致免疫抑制小鼠免疫功能的影响,探讨麦粒灸对免疫功能的调节机制。方法60只SPF级ICR小鼠随机分为空白组、模型组、中药组、麦粒灸组,各15只。空白组腹腔注射生理盐水0.2 ml,其余各组小鼠连续3 d腹腔注射环磷酰胺80 mg/kg造模;麦粒灸组在“大椎”“足三里”“三阴交”进行施灸,中药组给予贞芪扶正颗粒灌胃,每日1次连续7 d。后取材,HE法观察小鼠脾脏形态学变化,ELISA法检测血清中IL-2、IL-4、TNF-α、IFN-γ水平,Western blot检测IκBα、P-IκBα的蛋白表达水平。结果与空白组相比,模型组小鼠一般状况较差;白细胞(WBC)计数降低(P<0.01);脾脏组织出现很大程度损伤;血清中IL-4、IL-2水平降低,TNF-α、IFN-γ水平升高(P<0.01);脾脏组织P-IκBα蛋白的表达显著降低(P<0.01),而IκBα蛋白的表达高于空白组(P<0.01)。与模型组相比,麦粒灸组、中药组一般状况得到改善;WBC计数明显升高(P<0.01);脾脏损害程度有所减轻;血清中IL-4、IL-2水平升高,TNF-α、IFN-γ水平降低(P<0.01);P-IκBα蛋白的表达有不同程度的升高(P<0.01),IκBα蛋白的表达有所降低(P<0.01)。与中药组相比,麦粒灸组小鼠WBC计数略高(P<0.05),脾脏组织状况较好,IFN-γ蛋白水平较低(P<0.01)。结论麦粒灸可通过调节IκBα和P-IκBα的相对表达,影响NF-κB信号通路的活性,进而调节机体免疫应答反应,对环磷酰胺所致的免疫抑制起到缓解作用。This study was designed to investigate the effects of wheat grain moxibustion on the immune function of mice immunosuppressed by cyclophosphamide,and to delve into the potential mechanisms by analyzing factors related to NF-κB signaling pathway.Sixty SPF-grade ICR mice were randomly allocated into four groups:blank group,model group,traditional Chinese medicine group and wheat grain moxibustion group,with 15 mice in each group.Immunosuppression was induced in mice of the model,traditional Chinese medicine and wheat grain moxibustion groups by intraperitoneal injection of cyclophosphamide at 80 mg/kg daily for three days,while mice of the blank group received an equivalent volume of saline.The traditional Chinese medicine group was treated with oral administration of Zhēn QíFúZhèng granules,and the wheat grain moxibustion group was treated with wheat grain moxibustion at"Da Zhui,"bilateral"Zu San Li,"and bilateral"San Yin Jiao"once a day for seven consecutive days.After corresponding treatment,spleens were harvested from the mice of different groups for pathological examination,and enzyme-linked immunosorbent assay(ELISA)was utilized to measure the serum levels of IL-2,IL-4,TNF-αand IFN-γ.Additionally,Western blot analysis was conducted to assess the protein expression levels of IκBαand P-IκBαin spleen tissue.Compared with the blank group,mice in the model group exhibited poorer general conditions,significantly reduced white blood cell(WBC)count(P<0.01),extensive damage in spleen tissue,significant decreases in serum IL-4 and IL-2 concentrations,increases in TNF-αand IFN-γlevels(P<0.01),lower level of P-IκBαprotein in spleen tissue(P<0.01),and higher level of IκBαprotein(P<0.01).These changes mentioned above were reversed in mice of both the wheat grain moxibustion group and the traditional Chinese medicine group(P<0.01).Compared to the traditional Chinese medicine group,on the last day of treatment,the wheat grain moxibustion group showed slightly higher WBC counts(P<0.05),better tissue repair,an

关 键 词：麦粒灸 免疫抑制 NF-ΚB信号通路 IΚBΑ P-IκBα 

分 类 号：R245.81[医药卫生—针灸推拿学]