两株镰刀菌的生物学特性及培养性状研究  

Study on Biological and Cultural Characteristics of Two Strains of Fusarium

作　　者：李玲义 兰阿峰[1,2,3] 郭素芬 曹乐[1] 彭浩 LI Ling-yi;LAN A-feng;GUO Su-fen;CAO Le;PENG Hao(College of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723001,China;Qinba State Key Laboratory of Biological Resources and Ecological Environment(Incubation),Shaanxi University of Technology,Hanzhong 723001,China;Shaanxi Key Laboratory of Bio-resources,Hanzhong 723001,China)

机构地区：[1]陕西理工大学生物科学与工程学院,陕西汉中723001 [2]陕西理工大学秦巴生物资源与生态环境省部共建国家重点实验室(培育),陕西汉中723001 [3]陕西省资源生物重点实验室,陕西汉中723001

出　　处：《江西农业学报》2024年第10期35-46,69,共13页Acta Agriculturae Jiangxi

基　　金：陕西省重点研发计划一般项目——农业领域项目(2023-YBNY-091);陕西省教育厅科技计划项目(20JS024);陕南秦巴山区生物资源综合开发协同创新中心项目(SLGPT2019KF02-01)。

摘　　要：通过研究菌株Zb-21[腐皮镰刀菌(Fusarium solani)]和WX-6[三线镰刀菌(Fusarium tricinctum)]的生物学特性及培养特性,以明确其生长规律。生物学特性结果表明,光照条件对镰刀菌生长状态影响显著,菌株Zb-21在pH值为7、温度为20℃时生长最快,在30℃时产孢量及孢子萌发率最高;菌株WX-6在pH值为6~10、温度为20℃时生长最快、产孢量及孢子萌发率最高。培养特性结果表明,菌株Zb-21在以D-麦芽糖为碳源,无氮源,硫酸镁为微量元素,Czapek培养基中加维生素B1时菌丝生长最快;在以蔗糖为碳源,牛肉膏为氮源,微量元素为硫酸亚铁、加维生素C,培养基为CPDA时产孢量最高;在以葡萄糖为碳源,硫酸铵为氮源,微量元素为硫酸亚铁,加入维生素C,培养基为CA时分生孢子萌发率最高。菌株WX-6在以蔗糖为碳源,酵母浸粉为氮源,微量元素为硫酸镁,无维生素,培养基为Czapek或CA时菌丝生长最快;在以β-环糊精为碳源,微量元素为硫酸镁,复合维生素B,培养基为CPDA时产孢量最高;在以葡萄糖为碳源,蛋白胨为氮源,微量元素为硫酸镁,加入维生素B1,培养基为CPDA时分生孢子萌发率最高。在含磷酸盐的供试培养基中,菌株Zb-21在琼脂与磷酸盐分开灭菌时菌丝生长最快,CPDA培养基共同灭菌时产孢量最高;菌株WX-6在琼脂与磷酸盐共同灭菌的Czapek培养基中菌丝生长最快,CPDA培养基共同灭菌时产孢量最高。The biological and cultural characteristics of Zb-21(Fusarium solani)and WX-6(Fusarium tricinctum)were studied to understand their growth patterns.The results of the biological characteristics showed that light conditions had a significant influence on the growth state of Fusarium.The strain Zb-21 exhibited the greatest growth rate at pH value 7 and 20℃,while the highest spore production and spore germination rate were observed at 30℃.In contrast,the strain WX-6 demonstrated the greatest growth rate and the highest spore production and spore germination rate at pH value 6~10 and 20℃.The results of the cultural characteristics showed that Zb-21 exhibited the most rapid mycelial growth when the carbon source was D-maltose,in the absence of a nitrogen source,with magnesium sulphate serving as a trace element,and vitamin B1 incorporated into the Czapek medium.Conversely,the highest spore production was observed when sucrose was served as the carbon source,beef extract acted as the nitrogen source,ferrous sulphate was present as a trace element,vitamin C was added,and the medium was CPDA.The highest spore germination rate was observed when the carbon source was glucose,the nitrogen source was ammonium sulphate,the trace element was iron sulphate,and the medium was CA.The WX-6 strain exhibited the most rapid mycelial growth when sucrose was employed as the carbon source,yeast infusion powder as the nitrogen source,magnesium sulphate as the trace element,and no vitamins were added,and the medium was either Czapek or CA.The highest sporulation rate was achieved whenβ-cyclodextrin was the carbon source,magnesium sulphate was the trace element,vitamin B complex was used,and the mediu was CPDA.Additionally,the highest germination rate was observed when glucose was the carbon source,peptone was the nitrogen source,magnesium sulphate was the trace element,vitamin B1 was added,and CPDA was the medium.The test media containing phosphate demonstrated that Zb-21 exhibited the most rapid mycelial growth when sterilised sepa

关 键 词：镰刀菌 生物学特性 培养条件 产孢 

分 类 号：S182[农业科学—农业基础科学]