饲料小檗碱对高脂饲料斑马鱼生长性能、抗氧化能力、脂质代谢和肠道健康的影响  

Effects of Dietary Berberine on Growth Performance,Antioxidant Capacity,Lipid Metabolism and Intestinal Health in Zebrafish Fed High-fat Diet

作　　者：胡俊 熊玲芳 左妍斐 邢磊 林瑜婷 HU Jun;XIONG Ling-fang;ZUO Yan-fei;XING Lei;LIN Yu-ting(Zhanjiang Yuehai Aquatic Organisms Co.,Ltd.,Zhanjiang 524057,China)

机构地区：[1]湛江粤海水产生物有限公司,广东湛江524057

出　　处：《江西农业学报》2024年第10期54-62,共9页Acta Agriculturae Jiangxi

基　　金：湛江市级项目“高效海水养殖水体调控益生菌筛选、评估及应用研究”(2023A01054)。

摘　　要：以5%脂肪水平饲料作为对照组,11%脂肪水平饲料作为高脂组,并在高脂组的基础上添加100、200、300 mg/kg梯度的小檗碱,共配置5组试验饲料,分别记作LL、HL、HL100、HL200、HL300组。选取450尾斑马鱼随机分为5组,每组3个重复,每个重复30尾,投喂8周,结果表明:各处理组斑马鱼的生长性能无显著性差异,但高脂饲料对斑马鱼的肝脏和肠道健康产生了显著的负面影响,与HL相比,HL300组斑马鱼肝脏的T-CHO、TG、LDL-C含量,以及SOD、CAT、GSH、GPX、HL、LPL、TL酶活性均得到了显著提高,MDA和HDL-C含量则显著降低;添加200~300 mg/kg小檗碱能有效减少肝脏脂肪的富集,并增加肠道绒毛的高度;此外,小檗碱显著降低了肠道放线菌门和分枝杆菌属的丰度,其中300 mg/kg小檗碱处理显著增加了拟杆菌属的相对丰度、显著降低了罗尔斯通氏菌属的相对丰度。综上所述,小檗碱对斑马鱼促生长的作用不显著,但高脂饲料中添加300 mg/kg小檗碱可显著改善斑马鱼肝脏的抗氧化能力、肝脏脂质代谢、肠道健康。Five diets with 5%fat diet(CK),11%fat diet(high-fat group),and three 11%fat diets supplemented with 100,200,and 300 mg/kg of berberine were prepared and recorded as LL,HL,HL100,HL200 and HL300 groups,respectively.A total of 450 zebrafish were randomly divided into 5 treatment groups with 3 replicates per group and 30 fish per replicate for 8 weeks.The results showed that there were no significant differences in growth performance among all groups,but the high-fat diet(HL)had significant negative effects on liver and intestinal health.Compared with the HL group,the contents of T-CHO,TG and LDL-C and the activities of SOD,CAT,GSH,GPX,HL,LPL and TL in liver of zebrafish in the HL300 group were significantly increased,while the contents of MDA and HDL-C in liver were significantly decreased.Supplementation of 200~300 mg/kg berberine in a high-fat diet can effectively reduce the enrichment of liver fat and increase the height of intestinal villi.In addition,dietary berberine significantly decreased the relative abundance of Actinobacteria and Mycobacterium in the intestines of zebrafish fed high-fat diets,while supplementation with 300 mg/kg berberine significantly increased the relative abundance of Bacteroides,and significantly decreased the relative abundance of Rolstonia.In conclusion,dietary berberine had no significant growth-promoting effect on zebrafish fed with high-fat diets,but supplementation of 300 mg/kg berberine in high-fat diets could significantly improve liver antioxidant capacity,liver lipid metabolism and intestinal health of zebrafish.

关 键 词：斑马鱼 小檗碱 高脂 脂质代谢 肠道微生物 

分 类 号：S963.73[农业科学—水产养殖]