初始苯酚浓度对生物膜反应器降解苯酚及胞外聚合物生成的影响  

Effect of initial phenol concentration on phenol degradation and extracellular polymer generation in a biofilm reactor

作　　者：宋文瑄 董羽吉 解留安 余蕾 李静 廖涵乐 宋慧婷 SONG Wenxuan;DONG Yuji;XIE Liuan;YU Lei;LI Jing;LIAO Hanle;SONG Huiting(Hubei Key Laboratory of Regional Development and Environmental Response,Faculty of Resources and Environmental Science,Hubei University,Wuhan 430062,China;State Key Laboratory of Biocatalysis and Enzyme Engineering,School of Life Sciences,Hubei University,Wuhan 430062,China)

机构地区：[1]湖北大学资源环境学院,区域开发与环境响应湖北省重点实验室,湖北武汉430062 [2]湖北大学生命科学学院,省部共建生物催化与酶工程国家重点实验室,湖北武汉430062

出　　处：《湖北大学学报（自然科学版）》2024年第6期894-901,共8页Journal of Hubei University：Natural Science

基　　金：国家重点研发计划(2020YFA0908400、2019YFA090500);湖北省重点研发计划(2020BBA056)资助

摘　　要：采用生物膜反应器处理模拟高浓度含酚废水,考察初始苯酚浓度变化对含酚废水处理效果及生物膜胞外聚合物(extracellular polymeric substances,EPS)的影响。初始苯酚浓度由500 mg·L^(-1)逐渐提高至1500 mg·L^(-1),反应器总运行时间为38 d。实验结果表明:随着初始苯酚浓度的提高,苯酚和COD去除率呈先升后降的趋势,初始苯酚浓度范围为847.18~940.06 mg·L^(-1)时苯酚和COD去除率最高,60 h内苯酚和COD去除率分别达到100%和66.47%。高浓度初始苯酚的加入使微生物分泌更多的EPS,EPS含量先增加后维持稳定。初始苯酚浓度达到1325.42 mg·L^(-1)时,EPS、PS(polysaccharide)、PN(protein)达到最大值,分别为97.63 mg·g^(-1)(VSS)、42.82 mg·g^(-1)(VSS)、54.81 mg·g^(-1)(VSS)。初始苯酚浓度处于较高浓度范围(847~1481 mg·L^(-1))时,该反应器仍能较好地适应。以期为复合式生物膜法处理苯酚废水的工艺优化和机理研究提供指导。A biofilm reactor was used to treat high concentration phenol wastewater.The effect of initial phenol concentration variation on the treatment effect of phenol wastewater and extracellular polymeric substances(EPS)of biofilm was investigated.The initial phenol concentration of 500 mg·L^(-1) gradually increased to 1500 mg·L^(-1) within 38 days.The results showed that:with the increase of initial phenol concentration,the removal rates of phenol and COD increased firstly and then decreased.When the initial phenol concentration was within 847.18 and 940.06 mg·L^(-1),the removal rates of phenol and COD reached the maximum,and phenol could be completely degraded after 60 h with the COD removal rate of 66.47%.Initial phenol concentration had little effect on extracellular polymer secretion,which increased firstly and then remained stably.High concentration of phenol stimulated microorganisms to secrete more EPS.When the initial phenol concentration reached 1325.42 mg·L^(-1),EPS,PS and PN reached the maximum,which were 97.63 mg·g^(-1)(VSS),42.82 mg·g^(-1)(VSS)and 54.81 mg·g^(-1)(VSS),respectively.When the initial phenol concentration was in the high concentration range(847~1481 mg·L^(-1)),the reactor still adapted well.It is to provide guidance for the process optimization and mechanism research of phenol wastewater treatment by composite biofilm process.

关 键 词：生物膜 苯酚废水 降解 胞外聚合物 

分 类 号：X703.1[环境科学与工程—环境工程]