Identification and Antibacterial Activity Study of a Terrestrial Strain of Brevibacterium aureus 431  

作　　者：Jiedan Liao Junxiang Wei Yingwen Chen Yuchao Lin Hongyu Gao Yuchen Liu Danmin Zheng Guangwen Lai Weiquan Liang Wanjun Gu Shuzhen Zhou 

机构地区：[1]School of Animal Science and Technology,Foshan University,Foshan 528225,Guangdong Province,China [2]Foshan City Nanhai Eastern Along Pharmaceutical Co.,Ltd,Foshan 528234,Guangdong Province,China

出　　处：《Journal of Clinical and Nursing Research》2024年第10期231-242,共12页临床护理研究（英文）

基　　金：Self-funded Science and Technology Innovation Project of Foshan City(220001005797);Foshan University Innovation and Entrepreneurship Training Program for College Students in 2023;Foshan City Social Science Planning Project(2024-GJ037);Basic and Applied Basic Research Foundation of Guangdong Province(2022A1515140052);Innovation Project of Guangdong Graduate Education(2022JGXM129,2022JGXM128,2023ANLK-080)。

摘　　要：This study isolated and purified strain 431 from an animal probiotic product.Through staining and microscopic examination,colony morphology analysis,biochemical reaction tests,and 16S rDNA sequence alignment,the strain was identified and named Brevibacterium aureus 431.The study focused on the production of biosurfactants by strain 431,and antibacterial activity tests were conducted on the strain and its secondary metabolites.The results showed that strain 431 exhibited no resistance to 10 commonly used drugs,and its concentrated secondary metabolites were highly sensitive to the indicator bacterium Escherichia coli.Oral administration of strain 431 to BALB/c mice resulted in normal mental state,diet,and bowel movements,with no signs of illness or death,indicating that strain 431 is highly safe and non-pathogenic to mice.The study suggests that Brevibacterium aureus 431 has significant research value as a new source of actinomycetes and that its secondary metabolites have potential application value in the development of antibacterial drugs.

关 键 词：Brevibacterium aureus 431 Isolation and identification Genetic evolution analysis Antibacterial activity Drug susceptibility test Safety test 

分 类 号：TG1[金属学及工艺—金属学]