披碱草属4个牧草品种苗期抗寒性综合评价  

Comprehensive evaluation of cold resistance of four Elymus forage varieties at the seedling stage

作　　者：何永涛 胡宇[1] 段慧荣[1] 韩军 梁欢欢 田福平[1,2] HE Yongtao;HU Yu;DUAN Huirong;HAN Jun;LIANG Huanhuan;TIAN Fuping(Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences/The Lanzhou Scientific Observation and Experiment Field Station of Ministry of Agriculture for Ecological System in Loess Plateau Areas,Lanzhou 730050,Gansu,China;College of Ecology,Lanzhou University/State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems,College of Ecology,Lanzhou 730000,Gansu,China)

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所/农业部兰州黄土高原生态环境重点野外科学观测试验站,甘肃兰州730050 [2]兰州大学生态学院/草种创新与草地农业生态系统全国重点实验室,甘肃兰州730000

出　　处：《草业科学》2024年第10期2377-2388,共12页Pratacultural Science

基　　金：兰州大学“双一流”建设科研启动项目(561120227);抗旱节水型优质饲草筛选评价及生产示范项目(金科综2023-10);中国农业科学院创新工程项目“抗逆牧草育种与利用(25-LZIHPS-B02)”。

摘　　要：为比较披碱草属的抗寒性,选择同德短芒披碱草(Elymus breviaristatus‘Tongde’)、同德老芒麦(E.sibiricus‘Tongde’)、‘陇中1号’肥披碱草(E.excelsus‘Longzhong No.1’)和甘南垂穗披碱草(E.nutans‘Gannan’)4种披碱草作为试验材料,设置25、10、5和0℃4个温度梯度处理,测定不同处理下4个牧草品种的抗氧化酶活性、渗透调节物质含量、光合气体交换参数和叶绿素荧光参数等17个指标。利用隶属函数加权重的方法,综合评价4个牧草品种的抗寒性。结果表明,4个温度梯度下,同德短芒披碱草的T_(r)和G_(s)均差异显著(P<0.05),同德老芒麦的P_n、Ci和ETR值差异显著(P<0.05),甘南垂穗披碱草的qP、ΦPSⅡ和游离Pro积累量差异显著(P<0.05),‘陇中1号’肥披碱草的SS积累和REC差异显著(P<0.05)。综上可见,披碱草属4个牧草品种综合抗寒性由大到小表现为同德老芒麦>同德短芒披碱草>甘南垂穗披碱草>‘陇中1号’肥披碱草。本研究为利用披碱草属牧草建植高寒栽培草地和生态修复提供参考。To comprehensively compare the cold resistance of four forage grass species of Elymus,we used four Elymus forages(E.breviaristatus‘Tongde’,E.sibiricus‘Tongde’,E.excelsus‘Longzhong No.1’and E.nutans‘Gannan’)as experimental materials.We determined their antioxidant enzyme activity,osmoregulatory substance content,photosynthetic gas exchange parameters,and chlorophyll fluorescence parameters under four forage grass species under different treatments at four temperature gradients of 25,10,5,and 0℃.The cold resistance of these four forage grass species was evaluated comprehensively using the weighting method of the affiliation function.The results showed that under the four temperature gradients,the following parameters differed significantly(P<0.05):T_(r)and G_(s)of E.breviaristatus‘Tongde’,Pn,Ci,and ETR values of E.sibiricus‘Tongde’,qP,ΦPSⅡ,and free proline accumulation of E.nutans‘Gannan’,and SS accumulation and REC of E.excelsus‘Longzhong No.1’.The combined test results indicate that the comprehensive cold resistance ranking of the four Elymus forages is as follows:E.sibiricus‘Tongde’>E.breviaristatus‘Tongde’>E.nutans‘Gannan’>E.excelsus‘Longzhong No.1’.This study provides a reference for planting alpine artificial grassland and for ecological restoration using Elymus.

关 键 词：禾本科 抗寒性 抗氧化酶 光合作用 隶属函数 渗透调节物质 

分 类 号：S543.9[农业科学—作物学]