不同因素对药用大黄种子萌发和幼苗生长的影响  

Effects of Different Factors on Seed Germination and Seedling Growth of Rheum officinale

作　　者：王帆帆 唐涛 张路娜 张力斌 段媛媛 游景茂 WANG Fanfan;TANG Tao;ZHANG Luna;ZHANG Libin;DUAN Yuanyuan;YOU Jingmao(Institute of Chinese Herbel Medicine,Hubei Academy of Agricultural Science/Key Laboratory for Biology and Cultivation of Chinese Medicinal Materials of Ministry of Agriculture and Rural Affairs/Hubei Standardized Production of Traditional Chinese Medicine(GAP)Engineering Research Center,Enshi,Hubei 445000;Hubei Provincial Engineering Research Center of Under-forest Economy,Hubei Academy of Agricultural Science,Wuhan,Hubei 430064;Bureau of Science and Technology of Enshi Tujia and Miao Autonomous Prefecture,Enshi,Hubei 445000)

机构地区：[1]湖北省农业科学院中药材研究所,农业农村部中药材生物学与栽培重点实验室,湖北省中药材规范化生产(GAP)工程研究中心,湖北恩施445000 [2]湖北省农业科学院林下经济湖北省工程研究中心,湖北武汉430064 [3]恩施土家族苗族自治州科学技术局,湖北恩施445000

出　　处：《北方园艺》2024年第20期91-99,共9页Northern Horticulture

基　　金：恩施州科技计划资助项目(D20220055);国家现代农业产业技术体系建设专项资助项目(CARS-21);湖北省农业科学院青年科技基金资助项目(2023NKYJJ30);湖北省中央引导地方科技发展专项资助项目(2022BGE256);湖北省重点研发资助项目(2022BBA153)。

摘　　要：以三年生药用大黄种子为试材,采用三因素四水平的正交实验设计,研究了浸种时长、基质含水量和激素对药用大黄种子萌发及幼苗特性的影响,以期为药用大黄种苗繁育标准化规模化发展提供参考依据。结果表明:浸种时长为6 h、基质含水量为70%处理的种子出苗率最佳,T16(24 h,70%,赤霉素)的种苗叶面积、株高、地上质量和鲜质量均最佳,T8(12 h,70%,IAA)的种苗茎粗和叶片可溶性蛋白质含量最佳,T7(12 h,60%,水杨酸)的种苗根长和叶片过氧化物酶活性最佳,T10(18 h,50%,水杨酸)的种苗叶片丙二醛(MDA)含量最佳,T5(12 h,40%,6-BA)的种苗叶片超氧化物歧化酶(SOD)活性最佳。相关性分析和极差分析结果表明,药用大黄种苗繁育的理论最佳方案为浸种18 h、基质含水量保持70%和喷施水杨酸。Taking three-year-old seeds of Rheum officinale as the test materials,orthogonal experimental method with three factors and four levels was conducted to study the effects of soaking time,substrate water content and hormones on seed germination and seedling characteristics,in order to provide reference for the standardization and scale development of seedling breeding of R.officinale.The results showed that the seed emergence rate was the best when the soaking time was 6 hours and the substrate water content was 70%.The seedling leaf area,plant height,aboveground weight and fresh weight of T16(24 hours,70%,gibberellic acid)were the best.T8(12 hours,70%,IAA)had the best stem diameter and soluble protein content in leaves.The root length and leaf peroxidase activity treated with T7(12 hours,60%,salicylic acid)were the best.The content of malondialdehyde in leaves treated with T10(18 hours,50%,salicylic acid)was the best.Superoxide dismutase activity of seedling leaves treated with T5(12 hours,40%,6-BA)was the best.The results of correlation analysis and range analysis showed that the best theoretical scheme for seedling breeding of R.officinale was soaking seeds for 18 hours,maintaining 70%water content and spraying salicylic acid.

关 键 词：药用大黄 种苗 浸种时长 含水量 激素 

分 类 号：S35[农业科学—作物栽培与耕作技术]