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作 者:沈艳[1] 王维 何长生[1] 段倩倩 刘畅 刘华[1] 周迎春[1] 王倩[1] 王军[1] 苗文萍 朱良强[1] Shen Yan;Wang Wei;He Changsheng;Duan Qianqian;Liu Chang;Liu Hua;Zhou Yingchun;Wang Qian;Wang Jun;Miao Wenping;Zhu Liangqiang(Anhui Center for Animal Disease Prevention and Control,Hefei 230091,Anhui,China)
机构地区:[1]安徽省动物疫病预防与控制中心,安徽合肥230091
出 处:《中国动物检疫》2024年第10期13-17,共5页China Animal Health Inspection
摘 要:为了解安徽省3种引发猪群繁殖障碍疫病病毒的污染情况,2023年10—11月从安徽省16个市的101个屠宰场和20个无害化处理场收集了2110份猪淋巴结组织样品,采用荧光定量RT-PCR方法,对猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)、盖塔病毒(GETV)进行了检测。结果显示:CSFV、PRRSV、GETV的个体阳性率分别为0.19%、5.32%、5.86%,110份PRRSV阳性样品中,42份为类NADC30核酸阳性;屠宰场CSFV、PRRSV、GETV的场点阳性率分别为0.99%、41.58%、39.60%,PRRSV和GETV在皖北、皖南、皖中3个地区均有检出,而CSFV仅在皖南地区检出;无害化处理场CSFV、PRRSV、GETV的场点阳性率分别为10.00%、35.00%、10.00%;屠宰场和无害化处理场单一污染群占比分别为39.60%和25.00%,混合污染群占比分别为20.79%和15.00%。结果说明:安徽省猪瘟防控效果较好,而PRRSV和GETV污染面较广,且存在病原混合污染,类NADC30-PRRSV为安徽省田间流行的优势毒株。建议持续追踪PRRSV变异情况,做好疫苗免疫;加强对蚊、蠓等吸血节肢动物活动区域的消毒灭源工作,防控GETV传播;加大养殖密集地区的猪群疫病监控及调运监管力度。In order to investigate the contamination of three types of viruses causing pig reproductive disorders,2110 swine lymph node tissue samples were collected from 101 slaughterhouses and 20 rendering plants in 16 cities of Anhui Province from October to November 2023,to detect classical swine fever virus(CSFV),porcine reproductive and respiratory syndrome virus(PRRSV)and Getah virus(GETV)using fluorescence quantitative RT-PCR.The results showed that,for the three viruses,individual positive rates were 0.19%,5.32%and 5.86%,respectively,and 42 out of 110 PRRSV-positive samples were positive for NADC30-like nucleic acids;the farm/site positive rates of slaughterhouses were 0.99%,41.58%and 39.60%,respectively,PRRSV and GETV were detected in northern,southern and central Anhui,but CSFV was only detected in southern Anhui;the farm/site positive rates of rendering plants were 10.00%,35.00%and 10.00%,respectively;single contaminated herds on slaughterhouses and rendering plants accounted for 39.60%and 25.00%,respectively,and mixed contaminated herds were 20.79%and 15.00%,respectively.In conclusion,CSFV was effectively controlled,but PRRSV and GETV was widely distributed with mixed contamination,NADC30-like PRRSV was predominant in the field of Anhui Province.It was recommended to track all mutation of PRRSV in time and to conduct vaccination activities;to improve disinfection and source extermination against mosquitoes,midges and other blood-sucking arthropods in their living areas to prevent and control possible spread of GETV;and to increase monitoring for swine diseases and supervision over pig transportation in the areas with mass production of pigs.
关 键 词:猪瘟病毒 猪繁殖与呼吸综合征病毒 盖塔病毒 流行病学调查
分 类 号:S851.3[农业科学—预防兽医学]
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