北京市猫冠状病毒流行特征及S基因遗传进化分析  

Epidemiological Characteristics of Feline Coronavirus in Beijing City and Genetic Evolution Analysis of Its S Gene

作　　者：吴迪 李志军 苗东影 李刚 陈会玲 杜鹃 Wu Di;Li Zhijun;Miao Dongying;Li Gang;Chen Huiling;Du Juan(Beijing Center for Animal Disease Prevention and Control,Beijing 102629,China)

机构地区：[1]北京市动物疫病预防控制中心,北京102629

出　　处：《中国动物检疫》2024年第10期23-29,共7页China Animal Health Inspection

摘　　要：为了解北京市猫冠状病毒(feline coronavirus,FCoV)的生物型、血清型、遗传进化情况及其流行特征,对2023年在北京市动物医院收集的24份疑似FCoV感染猫的粪便或肛拭子样品进行RT-PCR检测、S基因扩增测序分析,并依据检测结果分析了来源猫年龄和采样季分布特征。结果显示:24份疑似样品中,检出11份FCoV阳性,其S基因核苷酸序列同源性为86.7%~98.5%,与日本、德国分离株同源性较高;11份阳性样品的血清型均为FCoV-I型,生物型均为猫肠道冠状病毒(FECV);猫只年龄与FCoV感染率呈负相关,季节变化与FCoV感染率不相关。结果表明:北京市2023年猫只感染的FCoV毒株属于同一血清型与生物型,且幼猫更易感染,一年四季皆可流行,同时提示毒株可能由入境宠物猫传入。因此在加强FCoV感染防控的同时,应重视出入境宠物猫FCoV的检测。In order to identify the biotypes,serotypes,genetic evolution and epidemiological characteristics of feline coronavirus(FCoV)in Beijing,24 fecal or anal swab samples collected from cats suspected of being infected with FCoV in Beijing Animal Hospital in 2023 were detected by RT-PCR,and followed by S gene amplification and sequencing,the age of the source cats and the distribution characteristics of seasons for sampling were analyzed based on the results.The results showed that 11 out of the 24 suspected samples were detected as FCoV positive,and the nucleotide sequence homology of their S genes ranged from 86.7%to 98.5%,which was highly homologous to the Japanese and German isolates;all the serotypes of the 11 positive samples were identified as FCoV-I,and all the biotypes were feline enterocoronavirus(FECV);the infection rate of FCoV was negatively related to the age of cats,but uncorrelated to seasonal variations.In conclusion,the FCoV strains infecting the cats in Beijing were classified as the same serotype and biotype prevalent throughout the year,especially kittens that were more susceptible,and the strains were likely to be introduced by entry pet cats that should be further monitored when FCoV infection was prevented and controlled.

关 键 词：猫冠状病毒 S基因 生物型 血清型 同源性 流行特征 

分 类 号：S852.65[农业科学—基础兽医学]