山东省部分地区奶牛金黄色葡萄球菌耐药性及毒力基因检测  

Detection of Drug Resistance and Virulence Genes of Staphylococcus aureus in Dairy Cattle in Shandong Province

作　　者：张淑爱 邵伟利 樊华 王倩倩 樊丽 Zhang Shu'ai;Shao Weili;Fan Hua;Wang Qianqian;Fan Li(Ningyang Animal Husbandry and Veterinary Development Service Center,Ningyang 271400,Shandong,China;Fushan Township Animal Husbandry and Veterinary Station,Ningyang 271400,Shandong,China;Tai'an Animal Husbandry and Veterinary Development Service Center,Tai'an 271000,Shandong,China)

机构地区：[1]宁阳县畜牧兽医事业发展服务中心,山东宁阳271400 [2]宁阳县伏山镇畜牧兽医站,山东宁阳271400 [3]泰安市畜牧兽医事业发展服务中心,山东泰安271000

出　　处：《中国动物检疫》2024年第10期30-35,共6页China Animal Health Inspection

基　　金：山东省科技特派员创新创业共同体泰安市奶牛产业服务团研究项目。

摘　　要：在全球抗生素耐药性危机下,耐药性金黄色葡萄球菌的出现,严重威胁着公众健康。为调查山东省奶牛场金黄色葡萄球菌流行情况及耐药性和毒力基因携带情况,2022—2023年采集济南、德州、泰安和临沂4个地区奶牛养殖场乳腺炎病牛奶样520份进行金黄色葡萄球菌分离鉴定,对分离得到的金黄色葡萄球菌分别采用药物敏感试验和PCR检测其耐药性及相关耐药和毒力基因。结果显示:共分离到57株金黄色葡萄球菌,分离率为11.0%(57/520),其中耐甲氧西林金黄色葡萄球菌(MRSA)的分离率为1.3%(7/520);分离的金黄色葡萄球菌对青霉素(93.0%,53/57)和复方新诺明(80.7%,46/57)耐药率较高,对万古霉素、多西环素、利奈唑胺最敏感,而对其他抗生素均产生了不同程度的耐药性,其中多重耐药率为45.6%(26/57)。从2株MRSA分离菌中检测到mecA耐药基因,未检测到mecC基因;检测到seb、sec、sed 3种毒力基因。结果表明,山东省4个地区奶牛场中普遍存在致病性金黄色葡萄球菌流行,且产生了一定的耐药性,对公共卫生具有潜在威胁,需要加强奶牛场乳腺炎防控。Staphylococcus aureus(S.aureus)with the capacity of drug resistance is emerging under global crisis of antibiotic resistance,posing a serious threat to public health.In order to investigate the prevalence of S.aureus and the carriage of virulence genes in dairy cattle farms in Shandong Province,from 2022 to 2023,520 milk samples were collected from cattle infected with mastitis in Jinan,Dezhou,Tai'an and Linyi cities to carry out isolation and identification of S.aureus,the drug resistance and relevant resistant and virulent genes of the isolates were detected by drug sensitive test and PCR,respectively.The results showed that 57 stains of S.aureus were isolated,with an isolation rate of 11.0%(7/520);the isolated strains were more resistant to penicillin(57/520),including methicillin-resistant S.aureus(93.0%(,MRSA 53/57))and cotrimoxazole with an isolation rate of 1.3%(80.7%,46/57),and the most sensitive to vancomycin,doxycycline and linezolid,but resistant to other antibiotics at different levels,with a multi-resistance rate of 45.6%(26/57).In addition to the virulence genes including seb,sec and sed,mecA resistance gene other than mecC was detected from 2 strains of MRSA isolates.In conclusion,pathogenic S.aureus was widely prevalent in dairy cattle farms in the 4 regions of Shandong Province and produced certain drug resistance,posing a potential threat to public health,and indicating a need to improve the prevention and control of mastitis in dairy cattle farms.

关 键 词：金黄色葡萄球菌 乳腺炎 药敏试验 肠毒素基因 耐药基因 

分 类 号：S851.3[农业科学—预防兽医学]