重组火鸡疱疹病毒活载体疫苗研究进展  

Research Progress on Recombinant Live Vector Vaccines of Herpesvirus of Turkey

作　　者：楚电峰 李扬 于晓璐 侯玉超 孙化露 Chu Dianfeng;Li Yang;Yu Xiaolu;Hou Yuchao;Sun Hualu(Qingdao Yebio Bioengineering Co.,Ltd.,National Key Laboratory of Veterinary Public Health and Safety,Shandong Provincial Animal Vaccine Technology Innovation Center,Qingdao Key Laboratory of Livestock and PoultryVaccine,Qingdao 266100,Shandong,China)

机构地区：[1]青岛易邦生物工程有限公司,兽医公共卫生安全全国重点实验室,山东省动物疫苗技术创新中心,青岛市畜禽疫苗重点实验室,山东青岛266100

出　　处：《中国动物检疫》2024年第10期79-84,共6页China Animal Health Inspection

基　　金：青岛市关键技术攻关及产业化示范类项目(22-3-4-xxgg-1-nsh)。

摘　　要：火鸡疱疹病毒因具有基因组庞大、复制非必需区域多、容纳外源基因能力强、安全性好、免疫持续时间长、相关基因操作技术成熟等众多优点成为活载体疫苗研究的热点。利用同源重组、细菌人工染色体、Fosmid和CRISPR等技术,研究人员成功构建了火鸡疱疹病毒的感染性克隆,并将其转染至易感细胞中拯救重组病毒。为使重组病毒高效表达外源蛋白,通常选择合适的插入位点、启动子和终止序列,并对外源基因序列进行优化处理。目前,以火鸡疱疹病毒为载体的重组疫苗在禽流感、新城疫、传染性法氏囊病、传染性喉气管炎、鹦鹉热衣原体病、艾美尔球虫病和鸭霍乱等多种疾病的预防和控制中展现出了广阔的应用前景。本文从火鸡疱疹病毒活载体疫苗的发展历程、构建方法与应用实践等方面进行综述,旨在为相关领域研究提供理论和实践指导。Herpesvirus of turkey(HVT),with the advantages of large genome,multiple nonessential replication regions,strong capacity to accommodate exogenous genes,good safety,long immune duration,mature gene manipulation technologies,etc.,has become a hot topic in live vector vaccine researches.Infectious clones of HVT were successfully constructed using homologous recombination,bacterial artificial chromosomes,Fosmid,CRISPR or other technologies,and transfected into susceptible cells to rescue the recombinant virus.In order to make the recombinant virus to efficiently express exogenous protein,suitable insertion site,promoter and termination sequence were usually selected,and the exogenous gene sequence was optimized.Recombinant vaccines taking HVT as vectors were used with wide prospects in the prevention and control of avian influenza,Newcastle disease,infectious bursal disease,infectious laryngotracheitis,Chlamydia psittaci infection,Eimeria coccidiosis infection,duck cholera and other diseases.Here,the history,construction method and application practice of live vector vaccines of HVT were reviewed,aiming to provide a theoretical and practical guidance for relevant researches.

关 键 词：火鸡疱疹病毒 活载体疫苗 构建方法 外源基因优化 实践应用 

分 类 号：S855.3[农业科学—临床兽医学]