海藻酸钠模拟体液环境下的凝胶转变及降解过程  

Gel Transformation and Degradation Process of Sodium Alginate in Simulated Body Fluid Environment

作　　者：李盈慧 张梦雪 周卜 刘书英 顾晓晓 王发合 吴则业 张德蒙 LI Yinghui;ZHANG Mengxue;ZHOU Bo;LIU Shuying;GU Xiaoxiao;WANG Fahe;WU Zeye;ZHANG Demeng(SKL(State Key Laboratory)of Marine Food Processing&Safety Control,Qingdao Bright Moon Seaweed Group Co.,Ltd.Qingdao 266000,China;Medical College,Qingdao Binhai University,Qingdao 266000,China;Qingdao Bright Moon Ocean Traditional Chinese Medicine Technology Co.,Ltd,Qingdao 266000,China)

机构地区：[1]青岛明月海藻集团有限公司海洋食品加工与安全控制全国重点实验室,山东青岛266000 [2]青岛滨海学院医学院,山东青岛266000 [3]青岛明月海洋中医药科技有限公司,山东青岛266000

出　　处：《烟台大学学报（自然科学与工程版）》2024年第4期373-379,共7页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：国家重点研发计划(2022YFC2402801);青岛市科技计划园区培育计划(23-1-5-yqpy-9-qy)。

摘　　要：为了探究海藻酸盐在人体内的凝胶转变规律和降解特性,在模拟体液环境下对海藻酸钠(SA)的凝胶化过程和降解过程进行系统研究。对于凝胶化过程,探究了凝胶形成环境以及SA的黏度、质量浓度、体积比等因素的影响;对于降解过程,探究了SA黏度、质量浓度、钙化时间等因素的影响。研究结果表明,在模拟体液环境中,凝胶生成率随时间几乎呈直线增长,且生成的凝胶质地均匀;相同的时间节点下(16 h),随着黏度的升高(从49 mPa·s到456 mPa·s),凝胶生成率从85.5%增加到100%;而随着溶液质量浓度的升高(从3 mg/mL到20 mg/mL),凝胶生成率达到100%所需要的时间从16 h增加至24 h。选取的112 mPa·s和196 mPa·s两种黏度规格形成的凝胶的降解规律无明显差异,差异在于初始形成的凝胶结构强弱,因此可根据注射部位所需的凝胶强度选择合适的黏度规格;凝胶消融降解速率与SA质量浓度有显著的相关性,质量浓度越高则降解越慢;相对于海藻酸钠凝胶(G_(SA-112))而言,OSA凝胶(G_(OSA-5))的降解速度更快,G_(OSA-5)在15 d内即可实现完全降解,而G SA则需要在第25天达到100%降解。因此,可通过SA黏度、质量浓度及氧化改性等参数实现体内植入凝胶消融降解的时间可控调节。To explore the gel transition and degradation characteristics of alginate in the human body,a systematic study was conducted on the gelation and degradation processes of sodium alginate(SA)under a simulated body fluid environment.For the gelation process,the influence of gel formation conditions,as well as the viscosity,mass concentration,and volume ratio of sodium alginate,were investigated.Regarding the degradation process,the effects of SA viscosity,mass concentration,and calcification time were explored.The experimental results show that in the simulated body fluid environment,the gel formation rate increased almost linearly,and the generated gel block exhibited uniform texture.At the 16 h mark,as the viscosity increased(from 49 mPa·s to 456 mPa·s),the gel formation rate rose from 85.5%to 100%.Meanwhile,as the solution mass concentration increased(from 3 mg/mL to 20 mg/mL),the time required to reach 100%gel formation increased from 16 to 24 h.There was no significant difference in the degradation pattern between gels formed with viscosities of 112 mPa·s and 196 mPa·s,but the difference lay in the strength of the initially formed gel structure.Therefore,the appropriate viscosity can be selected based on the gel strength required at the injection site.The gel degradation rate was strongly correlated with the SA mass concentration:higher concentrations lead to slower degradation.Compared to sodium alginate gel(G SA-112),oxidized sodium alginate gel(G_(OSA-5))degraded faster with G_(OSA-5)fully degrading within 15 days,while G_(SA-112)required 25 days to achieve complete degradation.Therefore,the degradation time of implanted gels in vivo can be controlled by adjusting SA viscosity,mass concentration and oxidative modification.

关 键 词：海藻酸钠 模拟体液 可注射性 氧化海藻酸钠 调控降解率 

分 类 号：O636[理学—高分子化学]