基于碳源转变的协同生物除磷模式构建及运行效能  

Establishment of Synergistic Biological Phosphorus Removal Pattern Based on Carbon Source Transformation and Its Performance

作　　者：张贤庆 俞小军[1] 张琨 赵龙 周怡君 于占洋 马娟[1,2,3] ZHANG Xian‑qing;YU Xiao‑jun;ZHANG Kun;ZHAO Long;ZHOU Yi‑jun;YU Zhan‑yang;MA Juan(School of Environmental and Municipal Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China;Gansu Wastewater Treatment Industry Technology Center,Lanzhou 730070,China;Engineering Research Center for Cold and Arid Regions Water Resource Comprehensive Utilization,Lanzhou 730070,China)

机构地区：[1]兰州交通大学环境与市政工程学院,甘肃兰州730070 [2]甘肃省污水处理行业技术中心,甘肃兰州730070 [3]寒旱地区水资源综合利用教育部工程研究中心,甘肃兰州730070

出　　处：《中国给水排水》2024年第19期1-8,共8页China Water & Wastewater

基　　金：国家自然科学基金资助项目(52060013);高校科研创新平台重大培育项目(2020CXPT-14);兰州交通大学基础研究拔尖人才项目(2022JC50)。

摘　　要：采用厌氧/好氧交替运行的SBR反应器,通过将进水碳源由葡萄糖逐步转变为混合氨基酸(谷氨酸、甘氨酸、脯氨酸和天冬氨酸),考察碳源转变对强化生物除磷(EBPR)系统运行效能及微生物菌群结构的影响,以构建协同强化生物除磷模式。结果表明,碳源转变有利于系统除磷,平均除磷率可达90%左右;但随着混合氨基酸比例的增加,氨氮去除率因氨基酸水解呈下降趋势。系统运行中期发生了污泥膨胀,及时调整后除磷性能得到完全恢复,氨氮去除量虽然有所增加,但去除率并未提高。16S rRNA高通量测序结果表明,当底物由葡萄糖转变为混合氨基酸后,发酵型聚磷菌(FPAOs)Tetrasphaera出现,且传统型聚磷菌(PAOs)Accumulibacter丰度大幅提高,硝化菌属丰度下降,这与系统的磷及氨氮去除性能表现一致,说明混合氨基酸碳源体系有利于协同强化生物除磷模式的构建,Tetrasphaera等发酵菌属可将氨基酸分解为利于Accumulibacter利用的小分子有机物,从而实现协同除磷。A sequencing batch reactor(SBR)operated under alternating anaerobic/aerobic conditions was employed to investigate the effects of carbon source switching from glucose to a mixture of amino acids(glutamic acid,glycine,proline,and aspartic acid)on the performance and microbial community structure of an enhanced biological phosphorus removal(EBPR)system,and to establish a synergistic EBPR pattern.The results indicated that the change in carbon source was beneficial for phosphorus removal,with an average phosphorus removal efficiency of about 90%.However,as the proportion of mixed amino acids increased,the ammonia nitrogen removal efficiency showed a declining trend due to the increased hydrolysis of amino acids.During the operation mid‑phase,sludge bulking occurred.Nevertheless,phosphorus removal performance recovered after system adjustments.Although the amount of ammonia nitrogen removed was improved slightly,the removal efficiency did not increase.High‑throughput 16S rRNA sequencing revealed that when the substrate was switched from glucose to mixed amino acids,fermentative polyphosphate‑accumulating organisms(FPAOs)like Tetrasphaera appeared,and the abundance of traditional PAOs,such as Accumulibacter,significantly increased,while the abundance of nitrifying bacteria decreased.These findings were consistent with the observed phosphorus and ammonia nitrogen removal performances,suggesting that the mixed amino acids were conducive to the establishment of synergistic EBPR pattern.Fermentative genera such as Tetrasphaera could biodegrade amino acids into small organic molecules,which were more easily utilized by Accumulibacter,thereby achieving synergistic phosphorus removal.

关 键 词：强化生物除磷 SBR 碳源转变 Tetrasphaera 协同作用 

分 类 号：TU992[建筑科学—市政工程]