On-chip dielectrophoretic single-cell manipulation  

作　　者：Zuyuan Tian Xihua Wang Jie Chen 

机构地区：[1]Department of Electrical and Computer Engineering,University of Alberta,Edmonton,AB T6G 1H9,Canada [2]Academy for Engineering&Technology,Fudan University,Shanghai 200433,China

出　　处：《Microsystems & Nanoengineering》2024年第4期87-114,共28页微系统与纳米工程（英文）

基　　金：by funding support from the Natural Sciences and Engineering Research Council(NSERC)and MITACS;supported by the China Scholarship Council(CSC)No.202006290002.

摘　　要：Bioanalysis at a single-cell level has yielded unparalleled insight into the heterogeneity of complex biological samples.Combined with Lab-on-a-Chip concepts,various simultaneous and high-frequency techniques and microfluidic platforms have led to the development of high-throughput platforms for single-cell analysis.Dielectrophoresis(DEP),an electrical approach based on the dielectric property of target cells,makes it possible to efficiently manipulate individual cells without labeling.This review focusses on the engineering designs of recent advanced microfluidic designs that utilize DEP techniques for multiple single-cell analyses.On-chip DEP is primarily effectuated by the induced dipole of dielectric particles,(i.e.,cells)in a non-uniform electric field.In addition to simply capturing and releasing particles,DEP can also aid in more complex manipulations,such as rotation and moving along arbitrary predefined routes for numerous applications.Correspondingly,DEP electrodes can be designed with different patterns to achieve different geometric boundaries of the electric fields.Since many single-cell analyses require isolation and compartmentalization of individual cells,specific microstructures can also be incorporated into DEP devices.This article discusses common electrical and physical designs of single-cell DEP microfluidic devices as well as different categories of electrodes and microstructures.In addition,an up-to-date summary of achievements and challenges in current designs,together with prospects for future design direction,is provided.

关 键 词：MANIPULATION utilize DIELECTRIC 

分 类 号：TN6[电子电信—电路与系统] O64[理学—物理化学]