男子橄榄球运动员肠道菌群特征和短链脂肪酸水平及其抗炎作用分析  

Gut microbiota characteristics,short-chain fatty acid contents and anti-inflammatory effect in male rugby players

作　　者：孟先峰[1] 李晟[2] 冯贵山 孟琰 张海彦 沙继斌[4] MENG Xianfeng;LI Sheng;FENG Guishan;MENG Yan;ZHANG Haiyan;SHA Jibin(Department of Cooperation and Development,Shandong Sports University,Ji'nan,Shandong 250102,China;不详)

机构地区：[1]山东体育学院合作发展处,山东济南250102 [2]山东体育学院竞技体育学院,山东济南250102 [3]山东体育学院研究生教育学院,山东济南250102 [4]山东体育学院运动与健康学院,山东济南250102

出　　处：《中国微生态学杂志》2024年第9期1016-1023,共8页Chinese Journal of Microecology

基　　金：山东省重点研发计划(2019GSF108155)。

摘　　要：目的观察男子橄榄球运动员所承受的强烈身体对抗及专项运动负荷对其肠道菌群结构特征与短链脂肪酸水平的影响,并观察其对机体炎症反应的可能影响。方法本试验招募男子橄榄球运动员30人作为试验组,招募非体育专业的在校男大学生15人作为对照组。分别按测试计划采集受试者空腹静脉血,收集粪便样本。采用酶联免疫吸附法测定受试者血浆中白介素(IL)-1、IL-6、IL-10、C反应蛋白(CRP)的水平。采用16S rDNA V3-V4区测序法比较受试者肠道菌群组成和结构。采用气相色谱-质谱联用法(GCMS)测定受试者粪便样本中短链脂肪酸(乙酸、丙酸、丁酸、戊酸)的含量。结果(1)试验组对象血浆中CRP水平显著低于对照组(P<0.01),其余指标未见显著差异。(2)与对照组相比,试验组对象粪便样本中普通拟杆菌(Bacteroides vulgatus)、伶俐粪球菌(Coprococcus comes)和食葡糖罗斯拜瑞菌(Roseburia inulinivorans)丰度均显著高于对照组(均P<0.05)。(3)与对照组相比,试验组对象肠道菌群Observed-species指数、Shannon指数均显著高于对照组(均P<0.05)。(4)与对照组相比,试验组对象粪便样本中乙酸、丙酸、丁酸浓度均显著升高(均P<0.05)。结论橄榄球运动员血浆中促炎因子CRP水平显著下降,提示其专项运动训练可发挥抗炎效应。橄榄球运动员所承受的专项运动负荷,可能会对其肠道微生物体系产生较大影响,其中菌群的改变可促使肠道中短链脂肪酸的生成增加。Objective To observe the changes of plasma inflammatory cytokines,gut microbiota structure and fecal shortchain fatty acid content in male rugby players.Methods A total of 30 male rugby players were recruited as the observation group,with 15 male college students not majoring in physical education as the control group.Fasting venous blood and fecal samples were collected.The plasma levels of IL-1,IL-6,IL-10 and CRP were determined with ELISA.The composition and structure of gut microbiota were compared using 16S rDNA sequencing within V3-V4 region.The contents of acetic acid,propionic acid,butyric acid and valeric acid in stool samples were determined by using gas chromatographymass spectrometry(GC-MS).Results(1)The concentration of CRP in the plasma in observation group was significantly lower than that in the control group(P<0.01).(2)Compared with the control group,the abundances of intestinal Bacteroides vulgatus,Coprococcus comes and Roseburia inulinivorans in observation group were significantly higher(all P<0.05).(3)Compared with the control group,the Observed species index and Shannon index of intestinal flora in observation group were significantly higher than those in the control group(all P<0.05).(4)Compared with the control group,the concentrations of acetic acid,propionic acid and butyric acid in the fecal samples in observation group were significantly increased(all P<0.05).Conclusion The concentration of proinflammatory cytokine CRP in the plasma of rugby players decreased significantly,suggesting that sports training may play a role in anti-inflammatory effects.A large amount of exercise,direct physical confrontation and intense special training may have a profound impact on the gut microbiota system of rugby players.The change in the intestinal flora components increased the production of short-chain fatty acids.

关 键 词：橄榄球 肠道菌群 短链脂肪酸 抗炎 

分 类 号：G804[文化科学—运动人体科学]