2023年浙江省湖州市A(H1N1)pdm09流感病毒血凝素分子特征分析  

Molecular characterization of hemagglutinin of A(H1N1)pdm09 influenza virus in Huzhou City,Zhejiang Province

作　　者：沈樟[1] 徐德顺[1] SHEN Zhang;XU Deshun(Huzhou Center for Disease Control and Prevention,Huzhou,Zhejiang 313000,China)

机构地区：[1]湖州市疾病预防控制中心,浙江湖州313000

出　　处：《上海预防医学》2024年第9期868-873,共6页Shanghai Journal of Preventive Medicine

摘　　要：【目的】分析浙江省湖州市2023年A(H1N1)pdm09流感病毒血凝素变异情况,分析其遗传进化特征。【方法】收集2家流感监测医院的呼吸道标本进行A(H1N1)pdm09流感病毒分离株和血凝素(HA)基因组测序,利用MEGA 7.0 BioAider等软件对流感病毒测序结果进行基因组相似性、基因遗传进化、抗原变异位点、受体结合位点、致病性、潜在糖基化位点进行分析。【结果】25株A(H1N1)pdm09流行株与疫苗株A/Wisconsin/67/2022基因核苷酸和氨基酸相似度分别为98.41%~99.22%和98.41%~99.36%。同属6B.1A.5a.2a进化谱系。HA蛋白中共发生19个氨基酸变异;其中在抗原表位Ca2簇发生S137P和R142K替换,潜在糖基化位共有7个点。与疫苗株相比,25株A(H1N1)pdm09流感病毒分离株潜在糖基化位点均未新增与缺失。25株分离株的在HA蛋白裂解位点区域未出现多个连续碱性氨基酸。【结论】2023年A(H1N1)pdm09流行株与疫苗株匹配度较好,接种推荐的疫苗对流感易感人群具有一定的保护作用,且目前湖州市pdm09H1N1流行株属于低致病性流感病毒特征。[Objective]To analyze the hemagglutinin variation and the genetic evolution of A(H1N1)pdm09 influenza virus in Huzhou City,2023.[Methods]Respiratory tract specimens from two influenza monitoring hospitals were collected for the isolation of A(H1N1)pdm09 influenza virus and HA genome sequencing.MEGA 7.0 and BioAider were utilized to analyze genetic similarity,genetic evolution,antigen mutation sites,receptor binding sites,pathogenicity,and potential glycosylation sites of the influenza virus sequencing results.[Results]The nucleotide and amino acid similarities between the 25 A(H1N1)pdm09 strains and the vaccine strain A/Wisconsin/67/2022 were 98.41%to 99.22%and 98.41%to 99.36%,respectively.These strains belonged to the 6B.1A.5a.2a evolutionary lineage.Notably,there were 19 amino acid variations in the HA protein,including substitutions of S137P and R142K in the epitope Ca2 cluster.Additionally,seven potential glycosylation sites were found in the HA protein,and no new or missing sites were observed in the 25 A(H1N1)pdm09 influenza virus isolates compared to the vaccine strains.Furthermore,no multiple continuous alkaline amino acids were detected in the HA cleavage site of the 25 isolates.[Conclusion]The A(H1N1)pdm09 pandemic strain in 2023 closely matches the vaccine strain,indicating good protection against the currently circulating A(H1N1)pdm09 influenza virus.The circulating A(H1N1)pdm09 strain in Huzhou is characterized as a low-pathogenic influenza virus.

关 键 词：流感病毒 A(H1N1)pdm09 血凝素 进化分析 

分 类 号：R183[医药卫生—流行病学]