4种消除M蛋白对血清尿酸检测干扰的方法比较分析  

A comparative analysis of four methods for eliminating M protein interference in serum uric acid detection

作　　者：许冬[1] 孟涛 陈剑[1] 李海霞[1] XU Dong;MENG Tao;CHEN Jian;LI Haixia(Department of Clinical Laboratory,Peking University First Hospital,Beijing 100034,China)

机构地区：[1]北京大学第一医院检验科,北京100034

出　　处：《临床检验杂志》2024年第10期728-732,共5页Chinese Journal of Clinical Laboratory Science

摘　　要：目的比较稀释法、聚乙二醇(PEG)沉淀法、超滤膜过滤法和手工计算法4种方法在清除M蛋白干扰尿酸检测中的差异,评估其在临床应用中的价值。方法对受M蛋白干扰的血清标本分别采用稀释法(去离子水和生理盐水)、PEG沉淀法、超滤膜过滤法和手工计算法进行尿酸检测和计算,以超滤膜过滤法的结果为参考值,比较4种清除M蛋白干扰的方法的价值。结果稀释法中,去离子水和生理盐水不同倍数稀释后结果与超滤法结果的相对偏倚分别为:3倍稀释-32.38%、-60.66%,5倍稀释-26.23%、-46.72%,10倍稀释-22.13%、-30.33%。PEG沉淀法与超滤法的相对偏倚为-3.28%,10例对照样本PEG沉淀前后的偏倚在-3.80%~2.34%之间。超滤膜过滤法的结果与患者原始结果相对偏倚为687.10%。手工计算法与PEG沉淀法和超滤膜过滤法的偏倚分别为-1.64%和1.69%,10例对照样本偏倚在-4.62%~0%之间。结论4种清除M蛋白干扰尿酸检测的方法在准确性、便捷性、经济性和实用性方面各有优劣。PEG沉淀法和超滤膜过滤法检测的准确度最高,但操作较繁琐,耗材成本较高。稀释法操作简单,实用性、经济性较好,但准确度较差。手工计算法需要对尿酸的检测参数及仪器检测原理有较好的掌握,其计算结果与超滤膜过滤法接近,方便经济快捷,可作为临床常规方法。Objective To compare the differences among dilution method,polyethylene glycol(PEG)precipitation method,ultrafil-tration membrane filtration method,and manual calculation method in eliminating M protein interference in uric acid detection and eval-uate their clinical application value.Methods The serum samples affected by M protein interference were subjected to uric acid detec-tion and calculation using dilution method(deionized water and physiological saline),PEG precipitation method,ultrafiltration mem-brane filtration method,and manual calculation method.The values of obtained from the four methods were compared,and the result of ultrafiltration membrane filtration method was used as the reference value.Results The relative biases between the results of the dilu-tion method and the ultrafiltration method after dferent dilutions of deionized water and physiological saline were as follows:-32.38%and-60.66%at 3-fold dilution,-26.23%and-46.72%at 5-fold dilution,and-22.13%and-30.33%at 10-fold dilution.The rela-tive bias between the PEG precipitation method and the ultrafiltration method was-3.28%.The bias before and after PEG precipitation in 10 control samples ranged from-3.80%to 2.34%.The relative bias between the results of the ultrafiltration membrane filtration method and the patient's original results was 687.10%.The biases of the manual calculation method compared to PEG precipitation method and the ultrafiltration membrane filtration method were-1.64%and 1.69%,respectively.The biases of 10 control samples were between-4.62%and 0%.Conclusion The four methods for clearing M protein interference in uric acid detection have their own ad-vantages and disadvantages in terms of accuracy,convenience,cost-effectiveness,and practicality.PEG precipitation method and ultra-filtration membrane filtration method have the highest accuracy,but they were more cumbersome to operate and have higher consumable costs.The dilution method was easy to perform and has good practicality and cost-effectiveness,but its accuracy was p

关 键 词：M蛋白 尿酸 干扰 消除方法 

分 类 号：R446[医药卫生—诊断学]