雷公藤多苷对IgA肾病大鼠肾脏保护及肾组织CB2R/NOX4/NLRP3表达的影响  

Effect of Tripterygium wilfordii polyglycosides on renal protection and expression of CB2R/NOX4/NLRP3 in renal tissue of IgA nephropathy rats

作　　者：陈晨晨 宋珂 丁樱[1,2] 赵文景[3] 张守琳[4] 宋纯东[1,2] CHEN Chen-chen;SONG Ke;DING Ying;ZHAO Wen-jing;ZHANG Shou-lin;SONG Chun-dong(Pediatric Hospital of the First Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou 450000,China;School of Pediatrics,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China;Beijing Traditional Chinese Medicine Hospital,Capital Medical University,Beijing 100010,China;Affiliated Hospital of Changchun University of Traditional Chinese Medicine,Changchun 130021,China)

机构地区：[1]河南中医药大学第一附属医院儿科医院,河南郑州450000 [2]河南中医药大学儿科医学院,河南郑州450046 [3]首都医科大学附属北京中医医院,北京100010 [4]长春中医药大学附属医院,吉林长春130021

出　　处：《中国药理学通报》2024年第11期2037-2041,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 82074493);河南省科教厅科技攻关项目(No 202102310494);河南省卫健委国家中医临床研究基地科研专项资助项目(No 2022JDZX 003);河南省中医药科学研究重大专项资助项目(No 2023ZYZD02);河南省中医药学科领军人才项目[豫卫中医函(2021)8号]。

摘　　要：目的探究雷公藤多苷对IgA肾病(IgA nephropathy,IgAN)大鼠肾脏保护及肾组织大麻素受体2(cannabinoids 2 receptor,CB2R)/NADPH氧化酶4(NADPH oxidase 4,NOX4)/核苷酸结合寡聚化结构域蛋白样受体蛋白3(NOD-like receptor protein3,NLRP3)表达的影响。方法“BSA+CCl 4+LPS”联合法复制IgAN大鼠模型,予雷公藤多苷干预。全自动生化分析仪检测24 h尿蛋白定量(24h-UTP)、尿红细胞计数(URBC)、血清白蛋白(ALB)、谷丙转氨酶(ALT)、血肌酐(Scr)、血尿素氮(BUN),光镜观察肾组织病理变化,免疫荧光观察系膜区IgA沉积情况;Western blot法检测肾脏组织中CB2R、NOX4、NLRP3的蛋白表达。结果与模型组相比,雷公藤多苷组大鼠肾脏病理损伤明显减轻,IgA电子致密物沉积减少;ALB水平升高,24h-UTP、URBC、ALT、Scr和BUN水平均降低;肾组织CB2R蛋白表达上升、NOX4、NLRP3蛋白表达降低。结论雷公藤多苷能有效改善IgAN大鼠肾脏损伤,其机制可能与调控CB2R/NOX4/NLRP3信号通路有关。Aim To investigate the effect of Tripterygium wilfordii polyglycosides(TWP)on renal protection and the expression of cannabinoid 2 receptor/NADPH oxidase4/NOD like receptor protoin3(CB2R/NOX4/NLRP3)in renal tissue of IgA nephropathy(IgAN)rats.Methods The IgAN rat model was replicated using the“BSA+CCl 4+LPS”combined method,and intervention with TWP was administered.A fully automated biochemical analyzer was used to detect 24-hour urine protein quantification(24h-UTP),urine red blood cell count(URBC),serum albumin(ALB),alanine aminotransferase(ALT),serum creatinine(Scr),and blood urea nitrogen(BUN).The pathological changes in renal tissue were observed under light microscopy,and IgA deposition in the mesangial area was observed using immunofluorescence.The protein expressions of CB2R,NOX4 and NLRP3 in renal tissue were detected by Western blot.Results Compared with the model group,the pathological damage to the kidneys of rats in the TWP group was significantly reduced,and the deposition of IgA electron dense material was significantly reduced.The levels of ALB in rats treated with TWP increased,while the levels of 24h-UTP,URBC,ALT,Scr,and BUN all decreased.The expression of CB2R protein in renal tissue of rats treated with TWP increased,while the expression of NOX4 and NLRP3 proteins was reduced.Conclusion TWP can effectively improve renal injury in IgAN rats,and its mechanism may be related to the regulation of the CB2R/NOX4/NLRP3 signaling pathway.

关 键 词：IGA肾病 雷公藤多苷 CB2R/NOX4/NLRP3 内源性大麻素系统 氧化应激 炎症反应 

分 类 号：R285.5[医药卫生—中药学]