基于LOX-1/NOX2信号通路探讨槲皮素抑制中性粒细胞胞外诱捕网形成机制  

Mechanism of quercetin inhibiting neutrophil extracellular trap formation based on LOX-1/NOX2 signaling pathway

作　　者：刘思 罗纲 王君畅 应琳瑶 王严 肖琳 LIU Si;LUO Gang;WANG Jun-chang;YING Lin-yao;WANG Yan;XIAO Lin(Xiangya School of Public Health,Central South University,Changsha 410078,China)

机构地区：[1]中南大学湘雅公共卫生学院,湖南长沙410078

出　　处：《中国药理学通报》2024年第11期2047-2054,共8页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 82003460,82103841);湖南省自然科学基金资助项目(No 2021JJ40814);湖南省研究生科研创新项目(No 2024ZZTS0284)。

摘　　要：目的探讨溶血磷脂酰胆碱(lysophosphatidylcholine,LPC)诱导中性粒细胞胞外诱捕网(neutrophil extracellular traps,NETs)形成的分子机制及槲皮素的干预作用。方法使用LPC诱导大鼠原代中性粒细胞,构建NETs形成的体外模型并进行槲皮素干预,检测NETs形成情况及相关通路蛋白表达水平。结果100 mg·L^(-1) LPC可在体外诱导NETs形成,其与LOX-1/NOX2通路相关蛋白表达上调有关,槲皮素干预可有效降低LOX-1和NOX2蛋白表达水平并减少NETs形成。结论槲皮素通过调控LOX-1/NOX2信号通路抑制NETs形成,发挥对相关炎症性疾病的保护作用。Aim To investigate the molecular mechanism of lysophosphatidylcholine(LPC)-induced neutrophil extracellular trap formation and the intervening role of quercetin.Methods An in vitro model of NETs formation was constructed using LPC-induced primary rat neutrophils,and quercetin intervention was performed.The formation of NETs and the expression level of related pathway proteins were detected.Results 100 mg·L^(-1) LPC induced the formation of NETs in vitro,and NETs formation was associated with the up-regulation of LOX-1/NOX2 pathway protein expression.Quercetin intervention effectively decreased LOX-1 and NOX2 protein expression,then inhibiting NETs formation.Conclusions Quercetin inhibits the formation of NETs by regulating the LOX-1/NOX2 signaling pathway and exerting a protective effect against related inflammatory diseases.

关 键 词：炎症 溶血磷脂酰胆碱 中性粒细胞胞外诱捕网 槲皮素 LOX-1 NOX2 

分 类 号：R285[医药卫生—中药学]