Isolation of Trichoderma virens 6PS-2 and its effects on Fusarium proliferatum f. sp. Malus domestica MR5 related to apple replant disease in China  

作　　者：Haiyan Wang Weixiao Tang Yunfei Mao Shurui Ma Xuesen Chen Xiang Shen Chengmiao Yin Zhiquan Mao 

机构地区：[1]State Key Laboratory of Crop Biology,College of Horticultural Science and Engineering,Shandong Agricultural University,Tai'an,Shandong,271018,China

出　　处：《Horticultural Plant Journal》2024年第6期1291-1308,共18页园艺学报（英文版）

基　　金：supported by China Agriculture Research System of MOF and MARA (Grant No.CARS-27);Shandong Agricultural Major Applied Technology Innovation Project (Grant No.SD2019ZZ008);Qingchuang Science and Technology Support Project of Shandong Colleges and Universities (Grant No.2019KJF020);Natural Science Foundation of Shandong Province (Grant No.ZR2020MC131);the National Key Research and Development Program of China (Grant No.2020YFD1000201);Taishan Scholars Funded Project (Grant No.ts20190923);the National Natural Science Foundation of China (Grant No.32072510);the Fruit Innovation Team in Shandong Province,China (Grant No.SDAIT-06-07)。

摘　　要：Apple replant disease(ARD) is primarily caused by biotic factors that seriously inhibits the development of apple industry. Therefore, the use of biological control measures to inhibit the main pathogens(such as Fusarium spp.) that cause ARD is of great significance to the sustainable development of the apple industry. Trichoderma virens 6PS-2, which exhibited antagonism toward a variety of pathogens, was screened from the rhizosphere soils of healthy apple trees(Malus robusta) in different replanted orchards in the Yantai and Zibo Cities, Shandong Province, China. Its fermentation extract inhibited the growth of pathogenic Fusarium proliferatum f. sp. Malus domestica MR5, which was proportional to the concentration. These substances also increased the hairy root volume and growth of Arabidopsis thaliana lateral roots. The phenotype of Malus hupehensis seedlings and microbial community structure in rhizosphere soils in greenhouse experiment using Highthroughput sequencing were analyzed, and the field experiment with grafted apple trees were used for further verification. Compared with the application of potato dextrose broth(PDB) medium, application of 6PS-2 spore suspension directly to replanted soils could improve the growth of M. hupehensis seedlings as well as the elongation of grafted apple trees. Concomitant decreases in the gene copy number of Fusarium and increases in the culturable bacteria/fungi were also observed in the greenhouse and field experiments. The abundance of Trichoderma,Bacillus, and Streptomyces increased significantly, but that of Fusarium, Pseudarthrobacter, and Humicola decreased. The content of esters, phenols,furans, and amino acids in root exudates of M. hupehensis seedlings increased, which significantly inhibited the multiplication of Fusarium, but was positively correlated with Bacillus and Trichoderma. In summary, T. virens 6PS-2 not only directly inhibits the activity of pathogenic Fusarium but also secrets secondary metabolites with antifungal and growth-promoting potential. In

关 键 词：Apple replant disease Trichoderma virens 6PS-2 BIOCONTROL Secondary metabolites Spore suspension High-throughput sequencing 

分 类 号：S436.611[农业科学—农业昆虫与害虫防治]