Systematic analysis and functional verification of citrus glutathione S-transferases reveals that CsGSTF1 and CsGSTU18contribute negatively to citrus bacterial canker  

作　　者：Jia Fu Liyan Su Jie Fan Qiyuan Yu Xin Huang Chenxi Zhang Baohang Xian Wen Yang Shuai Wang Shanchun Chen Yongrui He Qiang Li 

机构地区：[1]Citrus Research Institute,Southwest University/Chinese Academy of Agricultural Sciences,Chongqing 400712,China [2]School of Biological and Environmental Engineering,Xi'an University,Xi'an,Shaanxi 710065,China [3]National Citrus Engineering Research Center,Chongqing 400712,China [4]National Citrus Improvement Center,Chongqing 400712,China

出　　处：《Horticultural Plant Journal》2024年第6期1309-1320,共12页园艺学报（英文版）

基　　金：funded by the National Key Research and Development Program of China (Grant No.2022YFD1201600);Natural Science Foundation of Chongqing (Grant No.cstc2020jcyj-msxmX1064);Earmarked Funds for the China Agriculture Research System (Grant No.CARS-26);Three-year Action Plan of Xi'an University (Grant No.2021XDJH41)。

摘　　要：Citrus bacterial canker(CBC) is resulted from Xanthomonas citri subsp. citri(Xcc) infection and poses a significant threat to citrus production.Glutathione S-transferases(GSTs) are critical in maintaining redox homeostasis in plants, especially in relation to abiotic and biotic stress responses. However, the function of GSTs in resisting CBC remains unclear. Here, citrus glutathione S-transferases were investigated applying a genome-wide approach. In total, 69 CsGSTs belonging to seven classes were identified, and the phylogeny, chromosomal distribution, gene structures and conserved motifs were analyzed. Several CsGSTs responded to Xcc infection, as observed in the upregulation of CsGSTF1 and CsGSTU18 in the CBC-sensitive ‘Wanjincheng' variety but not in the resistant ‘Kumquat' variety. CsGSTF1 and CsGSTU18 were localized at the cytoplasm. Transient overexpression of CsGSTF1 and CsGSTU18 mediated reactive oxygen species(ROS) scavenging, whereas the virus-induced gene silencing(VIGS) of CsGSTF1 and CsGSTU18 caused strong CBC resistance and ROS burst. The present study investigated the characterization of citrus GST gene family, and discovered that CsGSTF1 and CsGSTU18 negatively contributed to CBC through modulating ROS homeostasis. These findings emphasize the significance of GSTs in infection resistance in plants.

关 键 词：CITRUS Glutathione S-transferases(GSTs) Citrus bacterial canker(CBC) Xanthomonas citri subsp.citri(Xcc) ROS homeostasis 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]