Genome-wide analyses of mi RNAs in mycorrhizal plants in response to late blight and elucidation of the role of miR319c in tomato resistance  

作　　者：Xiaoxu Zhou Zhengjie Wang Chenglin Su Jun Cui Jun Meng Yushi Luan 

机构地区：[1]School of Bioengineering,Dalian University of Technology,Dalian,Liaoning 116024,China [2]School of Computer Science and Technology,Dalian University of Technology,Dalian,Liaoning 116024,China [3]College of Life Science,Hunan Normal University,Changsha,Hunan 410081,China

出　　处：《Horticultural Plant Journal》2024年第6期1371-1382,共12页园艺学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant Nos.32230091,32072592)。

摘　　要：Tomato(Solanum lycopersicum), an economically important vegetable crop cultivated worldwide, often suffers massive financial losses due to Phytophthora infestans(P. infestans) spread and breakouts. Arbuscular mycorrhiza(AM) fungi mediated biocontrol has demonstrated great potential in plant resistance. However, little information is available on the regulation of mycorrhizal tomato resistance against P. infestans.Therefore, microRNAs(miRNAs) sequencing technology was used to analyse miRNA and their targets in the mycorrhizal tomato after P.infestans infection. Our study showed a lower severity of necrotic lesions in mycorrhizal tomato than in nonmycorrhizal controls. We investigated 35 miRNAs that showed the opposite expression tendency in mycorrhizal and nonmycorrhizal tomato after P. infestans infection when compared with uninfected P. infestans. Among them, miR319c was upregulated in mycorrhizal tomato leaves after pathogen infection. Overexpression of miR319c or silencing of its target gene(TCP1) increased tomato resistance to P. infestans, implying that miR319c acts as a positive regulator in tomato after pathogen infection. Additionally, we examined the induced expression patterns of miR319c and TCP1 in tomato plants exposed to salicylic acid(SA) treatment, and SA content and the expression levels of SA-related genes were also measured in overexpressing transgenic plants. The result revealed that miR319c can not only participates in tomato resistance to P. infestans by regulating SA content, but also indirectly regulates the expression levels of key genes in the SA pathway by regulating TCP1. In this study, we propose a novel mechanism in which the miR319c in mycorrhizal tomato increases resistance to P. infestans.

关 键 词：TOMATO Arbuscular mycorrhiza fungi Phytophthora infestans miRNA 

分 类 号：S641.2[农业科学—蔬菜学]