3种杀虫剂对向日葵田棉铃虫毒力及其解毒酶活性的影响  

Influences of three insecticides on virulence and detoxification enzyme activity of Helicoverpa armigera from sunflower fields

作　　者：孟佩 帖展疆 马朝阳 张桂源 肖庆会 张学坤[2] MENG Pei;TIE Zhanjiang;MA Chaoyang;ZHANG Guiyuan;XIAO Qinghui;ZHANG Xuekun(Anhui Research Institute of Chemical Industry,Hefei 230041,China;College of Agriculture,Shihezi University,Shihezi 832003,China;Institute of Agricultural Science,10 th Division,Xinjiang Production and Construction Corps,Beitun 836000,China)

机构地区：[1]安徽省化工研究院,安徽合肥230041 [2]石河子大学农学院,新疆石河子832003 [3]新疆生产建设兵团第十师农业科学研究所,新疆北屯836000

出　　处：《安徽农学通报》2024年第21期77-81,共5页Anhui Agricultural Science Bulletin

基　　金：国家特色油料产业技术体系(CARS-14-1-12);新疆生产建设兵团财政科技计划项目(2021AB011)。

摘　　要：为筛选有效防治向日葵田棉铃虫的药剂,本研究利用点滴法测定了2种化学杀虫剂(200 g/L氯虫苯甲酰胺和10%甲维·茚虫威)和1种生防制剂(8 000 IU/mg苏云金杆菌)对3龄棉铃虫的室内毒力,并探究了防效较好的药剂对其乙酰胆碱酯酶(ACh E)、羧酸酯酶(Car E)、细胞色素P450酶(CYP450)和谷胱甘肽S-转移酶(GST)4种解毒酶活性的影响。结果显示,200 g/L氯虫苯甲酰胺悬浮剂、10%甲维·茚虫威悬浮剂和8 000 IU/mg苏云金杆菌悬浮剂对3龄棉铃虫的致死中浓度(LC_(50))分别为2.36、11.86 mg/L和131.73倍,其中200 g/L氯虫苯甲酰胺的室内杀虫效果最好。200 g/L氯虫苯甲酰胺亚致死浓度(LC_(25))处理3龄棉铃虫12、24和48 h后,其体内ACh E活性受到抑制(P<0.05),而Car E和CYP450的活性明显增强(P<0.05),GST活性变化不明显(P>0.05)。表明棉铃虫可能主要通过Car E和CYP450进行解毒代谢。研究结果为向日葵田棉铃虫的防治提供参考。In order to screen effective insecticides for control Helicoverpa armigerain in sunflower field,the indoor toxicity of two chemical insecticides(200 g/L Chlorobenzamide and 10%Metre-indocarb)and one biocontrol agent(8000 IU/mg Bacillus thuringiensis)to Helicoverpa armigera was determined by drop methodand.The effects of better control insecticide on the activities of acetylcholinesterase(AChE),carboxylesterase(CarE),cytochrome P450 enzyme(CYP450)and glutathione S-transferase(GST)were investigated.The results showed that the lethal concentration of 50%LC_(50) of 200 g/L Chlorobenzamide suspension 10%Metre-indocarb suspension,and 8000 IU/mg Bacillus thuringiensis suspension on third instar of Helicoverpa armigera were 2.36,11.86 mg/L and 131.74 times,respectively,and the indoor insecticidal effect of Chlorobenzamide was the best.After treatment with Chlorobenzamide sublethal concentrations(LC_(25))for 12、24 and 48 h,the activity of AChE was significantly inhibited(P<0.05),while the activities of CarE and CYP450 were significantly enhanced(P<0.05),the activity of GST was not significantly changed(P>0.05).These results indicated that the detoxification metabolism of Helicoverpa armigera may be mainly through CarE and CYP450.The results provided references for the control of Helicoverpa armigera in sunflower field.

关 键 词：棉铃虫 杀虫剂 毒力测定 解毒酶活性 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]