高效液相色谱法检测头发提取液中辅酶Q_(10)的含量  

Determination of Coenzyme Q_(10)in Hair Extract by High Performance Liquid Chromatography

作　　者：吕思敏 丁鸿燕 李明贵 吴铁 LYU Simin;DING Hongyan;LI Minggui;WU Tie(Coenzyme Q_(10)Research Institute,Guangdong Medical University·Guangdong Runhe Biological Technology Co.,Ltd.,Dongguan 523808,China;School of Pharmacy,Guangdong Medical University,Dongguan 523808,China)

机构地区：[1]广东医科大学·广东润和生物科技有限公司辅酶Q_(10)联合研究中心,广东东莞523808 [2]广东医科大学药学院,广东东莞523808

出　　处：《山东化工》2024年第19期158-160,165,共4页Shandong Chemical Industry

基　　金：广东医科大学·广东润和生物科技有限公司共建辅酶Q_(10)联合研究中心项目(2XX14002)。

摘　　要：目的:建立高效液相色谱法(HPLC)检测头发提取液中辅酶Q_(10)含量的方法。方法:HPLC检测辅酶Q_(10)采用Eclipse Plus C18(4.6 nm×250 nm,5μm)为色谱柱,V(甲醇)∶V(乙醇)=20∶80为流动相,流速为1 mL/min,检测波长为275 nm,柱温为30℃,进样体积为20μL,对本校18~23岁大学生头发中辅酶Q_(10)的含量进行检测分析。结果:辅酶Q_(10)在0.78125~12.5μg/mL的质量浓度范围内线性关系良好,回归方程为:Y=19.596X+0.1148,R2=0.9999,平均加标回收率为92.96%,RSD为5.80%。结论:该法具有良好的线性关系,重现性好、精密度高、准确度好,可用于头发提取液中辅酶Q_(10)的含量测定。Objective:To establish a high performance liquid chromatography(HPLC)method for the determination of coenzyme Q_(10) in hair extract.Methods:Coenzyme Q_(10) was determined by HPLC using Eclipse Plus C 18(4.6 nm×250 nm,5μm)column with V(methanol)∶V(ethanol)=20∶80 as the mobile phase,the flow rate was 1 mL/min,the detection wavelength was set at 275 nm,the column temperature was 30℃,and the sample volume was 20μL.The content of coenzyme Q_(10) in hair of college students aged 18~23 was detected and analyzed.Results:The linear relationship of Coenzyme Q_(10) was good in the concentration range of 0.78125~12.5μg/mL.The regression equation was Y=19.596X+0.1148,R 2=0.9999.The average recovery rate was 92.96%and RSD was 5.80%.Conclusion:The method has good linear relationship,good reproducibility,high precision and good accuracy,and can be used for the determination of coenzyme Q_(10) in hair extract.

关 键 词：高效液相色谱法 头发 辅酶Q_(10) 含量 

分 类 号：TQ460[化学工程—制药化工] O657.7[理学—分析化学]