半胱胺修饰铜配合物用于氯霉素的灵敏检测  

Cysteamine-modified copper complexes for sensitive detection of chloramphenicol

作　　者：殷梓瑞 田磊 宋易泽 冯艳骥 唐晓丹[1] YIN Zirui;TIAN Lei;SONG Yize;FENG Yanji;TANG Xiaodan(School of Chemical Engineering,University of Science and Technology Liaoning,Anshan 114051,China)

机构地区：[1]辽宁科技大学化学工程学院,辽宁鞍山114051

出　　处：《辽宁科技大学学报》2024年第4期273-280,共8页Journal of University of Science and Technology Liaoning

基　　金：辽宁省教育厅项目(LJKMZ20220654);2024年辽宁科技大学大学生创新创业训练计划(S202410146093)。

摘　　要：为了提高荧光探针法检测抗生素氯霉素(CAP)的灵敏度和稳定性,本文制备半胱胺修饰的铜配合物(CuCy-Cl)荧光探针。实验结果表明,CuCy-Cl具有优异的荧光稳定性,并对氯霉素表现出较高的选择性和优异的灵敏度。基于动态猝灭机制,实现对抗生素氯霉素的有效识别。CAP浓度(C_(CAP))在0.5~9μmol/L或10~90μmol/L范围内,F_(0)/F与C_(CAP)呈良好线性关系,检出限为0.128μmol/L。CuCy-Cl成功用于湖水和氯霉素滴眼液中CAP含量的荧光测定,加标回收率为97.4%~103.2%。To enhance the sensitivity and stability of the fluorometric detection of the antibiotic chloramphenicol(CAP),a fluorescent probe of copper complex modified with cysteamine(CuCy-Cl)was prepared.The experimental results indicated that CuCy-Cl exhibited excellent fluorescence stability and demonstrated high selectivity and outstanding sensitivity to chloramphenicol.Based on the dynamic quenching mechanism,the effective identification of antibiotic chloramphenicol was realized.In the range of 0.5~9μmol/L or 10~90μmol/L,F_(0)/F showed a good linear relationship with CCAP,and the detection limit was 0.128μmol/L.CuCy-Cl was successfully used for the fluorescence determination of chloramphenicol in lake water and eye drops with recoveries of 97.4%~103.2%.

关 键 词：半胱胺修饰铜配合物(CuCy-Cl) 荧光检测 氯霉素 动态猝灭 

分 类 号：O657.3[理学—分析化学]