Awake rabbit model of ischemic spinal cord injury with delayed paraplegia:The role of ambient temperature  

作　　者：Wang Yang Qian-qian Wu Lu Yang Yu-jie Chen Ren-qing Jiang Ling Zou Qing-shan Liu Guang-you Shi Jiang Cao Xiao-chao Yang Jian Sun 

机构地区：[1]School of Biomedical Engineering and Medical Imaging,Army Medical University,Chongqing,China 2 Army Health Service Training Base,Army Medical University,Chongqing,China 3 Department of Neurosurgery,Southwest Hospital,Army Medical University,Chongqing,China

出　　处：《Animal Models and Experimental Medicine》2024年第5期732-739,共8页动物模型与实验医学（英文）

基　　金：supported by the Science and Technology Research Project(KJQN202212805)of the Chongqing Education Commission;the Special Funding Project(2021XJS08)of Army Medical University。

摘　　要：Background:Paraplegia after spinal cord ischemia is a devastating condition in the clinic.Here,we develop an awake rabbit model of spinal cord ischemia with delayed paraplegia and explore the influence of ambient temperature on the outcomes after injury.Methods:A total of 47 male rabbits were involved in the present study.Transient spinal cord ischemia was induced by occluding the infrarenal abdominal aorta of awake rabbits at different ambient temperatures.To find the optimal conditions for developing delayed paraplegia,hindlimb motor function after ischemia was evaluated between experiments.Results:The onset and magnitude of ischemic injury varied with the ambient temperature maintained during the peri-i schemia period.More serious spinal cord injury occurred when ischemia was induced at higher temperatures.At 18°C,25-minute ischemia resulted in 74%of rabbits developing delayed paraplegia.At a temperature of 28°C or higher,most of the animals developed acute paraplegia immediately.While at 13°C,rabbits usually regained normal motor function without paraplegia.Conclusion:This awake rabbit model is highly reproducible and will be helpful in future studies of delayed paraplegia after spinal cord ischemia.The ambient temperature must be considered while using this model during investigation of therapeutic interventions.

关 键 词：ambient temperature delayed paraplegia rabbit model spinal cord injury spinal cord ischemia 

分 类 号：R651.2[医药卫生—外科学] R-332[医药卫生—临床医学]