艾菖香精油的抗菌活性研究  

Studies on the Antibacterial Activity of Ai ChangXiang Essential Oil

作　　者：危赛龙 冯洁[1] 冯春煜 WEI Sai-long;FENG Jie;FENG Chun-yu(School of Pharmacy,Guangxi Medical University,Nanning 530021,China;Guangxi DiTe Technology Co.,Ltd.,Nanning 530201,China)

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]广西地特科技有限公司,广西南宁530201

出　　处：《海峡药学》2024年第9期10-13,共4页Strait Pharmaceutical Journal

基　　金：国家自然科学基金(No.81960706)。

摘　　要：目的研究中药艾菖香精油的抗菌活性,为进一步开发利用中药复方精油提供理论支持。方法通过水蒸气蒸馏法从中药复方粗粉中提取出艾菖香精油,测量比重(ρ)及得率。用抑菌圈实验测定大肠杆菌、金黄色葡萄球菌、耐药型金黄色葡萄球菌、绿脓杆菌、伤寒杆菌、乙型副伤寒杆菌对艾菖香精油的敏感度,同时使用微量肉汤稀释法得到艾菖香精油对这6种细菌的最低抑菌浓度(MIC),以评价精油的抑菌作用。结果艾菖香精油的ρ=0.94 g/cm^(3),得率为1.30%;精油对大肠杆菌、金黄色葡萄球菌、耐药型金黄色葡萄球菌、绿脓杆菌、伤寒杆菌、乙型副伤寒菌的抑菌圈直径分别为27.0 mm、26.0 mm、25.7 mm、10 mm、25.7 mm和22.3 mm,相应的MIC分别为1.175 mg·mL^(-1)、2.350 mg·mL^(-1)、2.350 mg·mL^(-1)、9.400 mg·mL^(-1)、0.588 mg·mL^(-1)、2.350 mg·mL^(-1)。结论艾菖香精油对6种细菌均有抗菌活性,其中对伤寒杆菌和大肠杆菌的抗菌活性最佳。OBJECTIVE The antibacterial activity of Ai ChangXiang essential oil was studied to provide theoretical support for the further application and utilization of the essential oil of traditional Chinese herbal formula.METHODS The essential oil was extracted from the traditional Chinese me dicine crude powder by steam distillation and the specific weight and extracted yield were detected too.We tested the sensitivities of Escherichia Coli,Staphy lococcus aureus,drug-resistant Staphylococcus aureus,Pseudomo nas aeruginosa,Salmonella typhi and Bacillus paratyphosus to Ai ChangXi ang essential oil using the inhibition zone test.Additionally,we determined the minimum inhibitory con centration(MIC)of Ai ChangXiang essential oil against these 6 species of bacte ria using broth microdilution method to assess its antibacterial effect.ESULTS Theρ=0.94 g/cm^(3)and extracted yield was 1.30%.The size of the inhibition zone of essential oil against E.Coli,S.aureus,drug-resis tant S.aureus,P.aeruginosa,S.typhi and B.paratypho sus were 27.0 mm,26.0 mm,25.7 mm,10 mm,25.7 mm and 22.3 mm,and the corresponding MIC were 1.175 mg·mL^(-1),2.350 mg·mL^(-1),2.350 mg·mL^(-1),9.400 mg·mL^(-1),0.588 mg·mL^(-1)and 2.350 mg·mL^(-1),respectively.C ONCLUSION The efficacy of Ai ChangXiang essential oil against 6 species of bacteria was demonstrated,as well as its antibacterial effect on S.typhi and E.Coli was the best.

关 键 词：精油 抗菌活性 抑菌圈 最低抑菌浓度 

分 类 号：R285[医药卫生—中药学]