便携超声辅助肝脏穿透伤活动性出血模型的建立  

Establishing a Liver Penetrating Injury Active Bleeding Model Assisted by Portable Ultrasound

作　　者：豆梦杰 高农 孙灏 黄河山 姬云鹏 陈威[3] 吕发勤[2] Dou Mengjie;Gao Nong;Sun Hao;Huang Heshan;Ji Yunpeng;Chen Wei;Lyu Faqin(Postgraduate Training Base of the Third Medical Center of the PLA General Hospital of Jinzhou Medical University,Beijing 100039,China;Department of Ultrasound,Third Medical Center,PLA General Hospital,Beijing 100039,China;Department of Emergency,Third Medical Center,PLA General Hospital,Beijing 100039,China;School of Materials Science and Engineering,Tianjin University,Tianjin 300350,China)

机构地区：[1]锦州医科大学解放军总医院第三医学中心研究生培养基地,北京市100039 [2]解放军总医院第三医学中心超声科,北京市100039 [3]解放军总医院第三医学中心急诊科,北京市100039 [4]天津大学材料科学与工程学院,天津市300350

出　　处：《中国超声医学杂志》2024年第10期1165-1167,共3页Chinese Journal of Ultrasound in Medicine

基　　金：国家重点研发计划(No.2022YFB4703500)。

摘　　要：目的探讨在便携超声引导下构建肝脏穿透伤动物模型的可行性及效果。方法选用巴马猪12只,在便携超声引导下利用微创旋切设备在实验猪肝右叶较厚处进行穿刺致伤,即刻行常规超声及超声造影检查,并记录实验动物的基本生命体征。出血3 h后处死动物并解剖。结果12只实验动物均建模成功,较建模前血压降低,血氧饱和度降低,心率增快(P<0.05),符合失血性休克的表现。建模后超声造影均显示肝脏穿透伤区有造影剂外溢、浓聚,证实存在活动性出血。处死后大体病理证实肝脏穿透伤。结论应用便携超声引导经皮旋切的方法可以建立肝脏穿透伤活动性出血模型,该模型稳定性好,成功率高,为后续肝脏穿透伤的止血治疗提供实验依据。Objective This paper discussed the feasibility and effect of constructing an animal model of liver penetrating trauma under the guidance of portable ultrasound.Methods Twelve Bama pigs were selected.Under the guidance of portable ultrasound,a minimally invasive rotary cutting device was used to puncture the thick part of the right lobe of the experimental pig liver,and then conventional ultrasound and contrast-enhanced ultrasound were performed immediately,and the basic vital signs of the experimental animals were recorded.Three hours after hemorrhage,the animals were sacrificed and dissected.Results All the 12 experimental animals were successfully modeled,and their blood pressure and blood oxygen saturation were lower than those before modeling,and their heart rate was higher than that before modeling(P<0.05),which was consistent with the performance of hemorrhagic shock.After modeling,CEUS showed that there was extravasation and concentration of contrast agent in the penetrating injury area of the liver,which confirmed the existence of active bleeding.Penetrating liver injury was confirmed by gross pathology after death.Conclusions The liver penetrating injury active bleeding model can be established by the method of percutaneous cutting guided by portable ultrasound.The model has good stability and high success rate,which provides experimental basis for the subsequent hemostatic treatment of liver penetrating injury.

关 键 词：肝脏损伤 穿透性 介入超声 动物模型 

分 类 号：R445.1[医药卫生—影像医学与核医学] R657.3[医药卫生—诊断学]