1株牛源腹泻大肠杆菌耐药基因检测  

Detection of antibiotic resistance genes in a bovine diarrhea Escherichia coli strain

作　　者：于辰龙 康秀华 陈亮 冯万宇 兰世捷 苗艳 田晓鹏 张蕾 Yu Chenlong;Kang Xiuhua;Chen Liang;Feng Wanyu;Lan Shijie;Miao Yan;Tian Xiaopeng;Zhang Lei(Branch of Animal Husbandry and Veterinary,Heilongjiang Academy of Agricultural Sciences,Heilongjiang Qiqihar 161000;Animal Husbandry Station of Niujiayingzi Town,Harqin Banner Chifeng City,Inner Mongolia,Inner Mongolia Chifeng 024421;Animal Husbandry and Veterinary Work Station in Changyi District,Jilin City,Jilin Province,Jilin Jilin 132002)

机构地区：[1]黑龙江省农业科学研究院畜牧兽医分院,黑龙江齐齐哈尔161000 [2]内蒙古赤峰市喀喇沁旗牛家营子镇畜牧站,内蒙古赤峰024421 [3]吉林省吉林市昌邑区畜牧兽医工作总站,吉林吉林132002

出　　处：《现代畜牧兽医》2024年第8期6-8,共3页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：齐齐哈尔市科技计划创新激励项目(CNYGG-2023022)。

摘　　要：试验旨在分析牛源腹泻大肠杆菌耐药原因,并获悉16S rRNA甲基化酶基因携带情况。采用药敏试纸对腹泻大肠杆菌进行体外药物敏感试验,通过PCR检测分离株16S rRNA甲基化酶基因携带情况,给昆明小白鼠腹腔注射稀释菌液观测,发病死亡情况。结果显示,该株大肠杆菌携带rmtB耐药基因,对头孢他啶、头孢噻肟、多黏菌素、头孢吡肟敏感,对其余8种不同种类抗生素均表现耐药。试验小鼠在注射稀释菌液5 h后开始发病,48 h内全部死亡。研究表明,该牛源腹泻大肠杆菌对阿米卡星、卡那霉素等氨基糖苷类药物耐药,提示要合理规范使用抗生素。The experiment aimed to analyze the reasons for antimicrobial resistance in Escherichia coli causing diarrhea in cattle and to determine the carriage of 16S rRNA methyltransferase genes.The susceptibility of the diarrhea-causing Escherichia coli to antimicrobial agents was tested using antibiotic sensitivity test strips,and the carriage of 16S rRNA methyltransferase genes in the isolated strains was detected by PCR.Additionally,Kunming white mice were injected intraperitoneally with diluted bacterial solution to observe the onset and death from the infection.The results showed that the Escherichia coli strain carried the rmtB resistance gene and was sensitive to ceftazidime,cefotaxime,colistin,and cefoperazone,but resistant to other eight types of antibiotics.The test mice began to show symptoms five hours after injection with the diluted bacterial solution and all died within 48 hours.The study indicates that this bovine diarrhea Escherichia coli strain is resistant to aminoglycoside drugs such as amikacin and kanamycin,suggests the need for rational and standardized use of antibiotics.

关 键 词：牛源大肠杆菌 抗生素 16S rRNA甲基化酶 氨基糖苷类药物 耐药基因 

分 类 号：S852.61[农业科学—基础兽医学]