HPLC法测定杜仲叶提取物中京尼平苷酸、绿原酸、京尼平苷、芦丁含量  

Determination of iridoid glycosides,chlorogenic acid,loganic acid,and rutin in Eucommia folium extract by HPLC

作　　者：张志晖 姚浪群 董素军 Zhang Zhihui;Yao Langqun;Dong Sujun(Hebei Technology Innovation Center of Plant Extract Feed Additives,Hebei Ailu Bioengineering Co.,Ltd.,Hebei Langfang 065400)

机构地区：[1]河北省饲用植物提取物技术创新中心河北爱绿生物工程有限公司,河北廊坊065400

出　　处：《现代畜牧兽医》2024年第8期19-22,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

摘　　要：研究旨在建立高效液相色谱法同时测定杜仲叶提取物中尼平苷酸、绿原酸、京尼平苷、芦丁含量的方法。试验选择Agilent ZORBAX SB-C18色谱柱,以乙腈-0.4%磷酸溶液为流动相,流速1.0 mL/min,柱温30℃,进样量10μL,波长235 nm。结果显示:4种成分在检测范围中所呈现出的线性关系良好,平均加样回收率为100.8%~103.0%,相对标准偏差(RSD)为0.90%~2.37%。不同生产厂家的杜仲叶提取物中4种成分含量存在一定差异。研究表明,试验建立的方法操作简单、稳定可靠、重复性好,可以用于杜仲叶提取物的质量控制。The study aimed to establish a method for the simultaneous determination of loganic acid,chlorogenic acid,iridoid glycosides and rutin,in Eucommia folium extract by high-performance liquid chromatography(HPLC).The experiment utilized an Agilent ZORBAX SB-C18 column,with acetonitrile-0.4%phosphoric acid solution as the mobile phase,a flow rate of 1.0 mL/min,column temperature at 30℃,injection volume of 10μL,and detection wavelength at 235 nm.The results indicated that the four components exhibited good linearity within the tested range,with average spiked recovery rates of 100.8%to 103.0%and relative standard deviations(RSD)of 0.90%to 2.37%.There were certain differences in the content of the four components in Eucommia folium extract from different manufacturers.The study demonstrated that the established method is simple to operate,stable,reliable,and has good repeatability,making it suitable for quality control of Eucommia folium extract.

关 键 词：高效液相色谱法 杜仲叶提取物 京尼平苷酸 绿原酸 京尼平苷 芦丁 

分 类 号：S816[农业科学—饲料科学]