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作 者:王琳[1] 刘淼 刘素英[1] 董曦[1] 车祺 WANG Lin;LIU Miao;LIU Suying;DONG Xi;CHE Qi(Reproduction Medicine Center,Zhongshan Hospital,Fudan University,Shanghai 200032,China)
机构地区:[1]复旦大学附属中山医院生殖医学中心,上海200032
出 处:《中国生育健康杂志》2024年第6期509-513,F0004,共6页Chinese Journal of Reproductive Health
基 金:国家自然科学基金面上项目(82071643)。
摘 要:目的研究蒲公英提取物(T1口服液)对于卵巢功能减退的治疗作用,并初步探讨其机制。方法以绝经过渡期健康雌性小鼠为卵巢功能衰退的研究对象,青年雌性小鼠作为阳性对照,观察绝经过渡期小鼠饲养蒲公英提取物30 d后,卵巢窦状卵泡(AFC)的数量变化。同时培养人卵巢颗粒细胞系(KGN细胞),利用流式细胞技术、TUNEL法和细胞凋亡PCR芯片,比较蒲公英提取物孵育前后,KGN细胞的凋亡改变及差异性表达的基因。结果经蒲公英提取物饲养的绝经过渡期小鼠,卵巢窦状卵泡数量显著高于对照组(P<0.01)。且蒲公英提取物孵育后,KGN细胞凋亡率显著低于对照组(P<0.05)。细胞凋亡PCR芯片筛选出差异性表达的20个下调基因和4个上调基因(P<0.05)。结论动物模型中,蒲公英提取物可有效治疗雌性小鼠的卵巢功能减退,其机制可能是通过降低卵巢颗粒细胞的凋亡率。Objective To study the therapeutic effect of dandelion extract(Shawkea T-1)on ovarian hypofunction and its mechanism.Methods Menopausal transition mice were the intervention group for ovarian hypofunction,and healthy young female mice were the control group.The number of ovarian antral follicles in menopausal transition mice fed with dandelion extract for 30 days was observed.At the same time,human granulosa cell line(KGN)was cultured.The apoptosis of KGN cells was compared after incubation with dandelion extract using flow cytometry,TUNEL and apoptosis PCR array.Results The number of ovarian antral follicles in postmenopausal mice fed with dandelion extract was significantly higher than that in the control group(P<0.01).The apoptosis rate of KGN cells of dandelion extract culture group was significantly lower than that in control group(P<0.05).20 up-regulated genes and 4 down regulated genes were screened by apoptosis PCR chip(P<0.05).Conclusion In the animal model,dandelion extract can effectively treat the ovarian hypofunction of female mice.The underlying mechanism may be due to the decreasing apoptosis rate of granulosa cells.
关 键 词:卵巢功能减退 窦状卵泡 颗粒细胞 细胞凋亡 PCR芯片
分 类 号:R33[医药卫生—人体生理学]
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