基于转录组学筛选蓝莓花粉败育相关候选基因  

Screening of candidate genes related to pollen abortion of blueberry by transcriptomics

作　　者：杨芩 付燕[3] 刘雅兰 张婷渟 彭舒 邓洁 YANG Qin;FU Yan;LIU Ya-lan;ZHANG Ting-ting;PENG Shu;DENG Jie(College of Life and Health Science,Kaili University,Kaili,Guizhou 556000,China;Key Laboratory of Molecular Breeding and Variety Creation of Horticultural Plants for Mountain Features in Guizhou Province,Kaili,Guizhou 556000,China;Qiandongnan Vocational and Technical College,Kaili,Guizhou 556000,China)

机构地区：[1]凯里学院大健康学院,贵州凯里556000 [2]贵州山地特色园艺作物分子育种与品种创制重点实验室,贵州凯里556000 [3]黔东南民族职业技术学院,贵州凯里556000

出　　处：《西南农业学报》2024年第9期2018-2030,共13页Southwest China Journal of Agricultural Sciences

基　　金：凯里学院2022年农业硕士专业学位培育点专项课题(kysszx2022014);贵州省教育厅自然科学研究项目(黔教技[2022]068号);贵州省高等学校重点实验室项目(黔教技[2022]053号);凯里学院贵州省省级一流本科专业(园艺)、贵州省科技计划重点项目(黔科合基础[2019]1443号);凯里学院一流学科(园艺学)共同资助。

摘　　要：【目的】筛选蓝莓花粉败育相关基因,为其克隆、蓝莓花粉败育研究和育种提供参考依据。【方法】用Illumina NovaSeq 6000对来源于相同母本且花粉活力有显著差异的‘灿烂(BT)’‘杰兔(PM)’和‘粉蓝(PB)’3个兔眼蓝莓品种花粉的转录组进行测序,基于差异表达基因的基因本体数据库(GO)、Pathway显著性富集(KEGG)和蛋白家族数据库(Pfam)分析,筛选参与蓝莓花粉败育的相关基因。【结果】‘杰兔’花粉活力显著低于‘灿烂’和‘粉蓝’,‘灿烂’vs‘杰兔’与‘粉蓝’vs‘杰兔’2个对比组合分别筛选到2717和3208个差异表达基因。‘灿烂’vs‘杰兔’对比组合的2717个差异表达基因在GO、KEGG和Pfam数据库中分别已注释1124、1020和1791个。‘粉蓝’vs‘杰兔’对比组合的3208个差异表达基因在GO、KEGG和Pfam数据库中分别已注释1350、1144和2197个。对差异表达基因的GO和Pfam注释结果进一步筛选,获得与花粉败育相关的差异表达基因27个,其中,22个上调表达,5个下调表达。【结论】经GO和Pfam注释,花粉败育相关差异表达基因功能主要集中在花粉发育、花穗发育、孢粉素生物合成过程、花粉外壁形成、花药壁绒毡层细胞分化、细胞分裂素的正向调节、有丝分裂纺锤体组织调节和油菜素内酯介导信号途径等通路,可作为深入探索蓝莓花粉败育机制及筛选蓝莓花粉败育关键基因的参考依据。[Objective]The genes related to pollen abortion of blueberry were screened to provide reference for its cloning and related research and breeding.[Method]Illumina NovaSeq 6000 was used to sequence the transcriptomes of pollen from three rabbiteyed blueberry cultivars,‘Brightwell(BT)',‘Powderblue(PB)'and‘Premier(PM)',which were derived from the same female parent and had significant differences in pollen viability.The genes related to pollen abortion of blueberry were screened based on the Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)and Protein family(Pfam).[Result]The pollen viability of‘PM'was significantly lower than that of‘BT'and‘PB',and 2717 and 3208 differentially expressed genes were detected in the comparison combinations of‘BT'vs‘PM'and‘PB'and‘PM',respectively.There were 1124,1020 and 1791 differentially expressed genes in the GO,KEGG and Pfam databases of the 2717 differentially expressed genes in the comparison combination of‘BT'vs‘PM',while the 3208 differentially expressed genes of‘PB'vs‘PM'were annotated 1350,1144 and 2197 differentially expressed genes in GO,KEGG and Pfam databases,respectively.Further screening of GO and Pfam annotation results of differentially expressed genes revealed that 27 differentially expressed genes were related to pollen abortion,of which 22 genes were up-expressed and 5 genes were down-expressed.[Conclusion]The annotation results of GO and Pfam differentially expressed genes related to pollen abortion show that pollen development,inflorescence development,sporopollenin biosynthetic process,formation of pollen exine,cell differentiation of anther wall tapetum,positive regulation of cytokinesis,regulation of mitotic spindle organization,brassinosteroid mediated signal process and other pathways are closely related to pollen abortion in blueberry.It can be used as a reference for further exploring the mechanism of pollen abortion and mining the key genes of pollen abortion in blueberry.

关 键 词：蓝莓 花粉败育 转录组 基因筛选 育种 

分 类 号：S663.9[农业科学—果树学]