白背飞虱Hsp70基因的克隆及其对非生物胁迫的响应  

Cloning of Hsp70 gene in Sogatella furcifera and its response to abiotic stress

作　　者：许铁龙 周操 XU Tie-long;ZHU Cao(Guizhou Provincial Engineering Research Center of Medical Resourceful Healthcare Products,Guiyang Healtheare Vocational University,Guiyang 550081,China;College of Life Sciences,Chongqing Normal University,Chongqing 401331,China)

机构地区：[1]贵阳康养职业大学,贵州省药用资源康养产品工程研究中心,贵阳550081 [2]重庆师范大学生命科学学院,重庆401331

出　　处：《西南农业学报》2024年第9期2069-2076,共8页Southwest China Journal of Agricultural Sciences

基　　金：贵阳康养职业大学博士启动基金科研项目(贵康大K2022-6)。

摘　　要：【目的】探明热休克蛋白Hsp70基因在白背飞虱适应非生物[杀虫剂、高温/低温和紫外线A(UV-A)]胁迫中的作用,为白背飞虱绿色防控提供参考。【方法】基于白背飞虱的基因组和转录组数据,克隆白背飞虱热休克蛋白Hsp70基因并对其进行鉴定,同时采用RT-qPCR测定其在杀虫剂、高温/低温和UV-A胁迫下的转录水平。【结果】克隆获得1个Hsp70基因,命名为SfHsp70-1,其全长序列为2322 bp,开放阅读框为2001 bp,编码666个氨基酸残基,分子量为72.91 kDa,理论等电点为5.11。SfHsp70-1氨基酸序列中包含3个Hsp70家族蛋白的保守结构域,且在C端具有内质网Hsp70的保守序列“HDEL”。噻虫嗪亚致死浓度表现出显著的剂量效应,LC_(10)胁迫能够诱导SfHsp70-1基因的转录水平显著升高,为对照(丙酮处理)的2.59倍;而LC_(25)胁迫对SfHsp70-1基因的转录水平无显著影响。10℃(30 min)、20℃(30,60 min)和40℃(10,30,60 min)胁迫均能诱导SfHsp70-1基因的转录水平显著提高,依次较对照(25℃)显著提高77.24%、62.00%、85.89%、138.96%、75.67%和61.10%。UV-A(300μW/cm~2)胁迫显著抑制SfHsp70-1基因的转录水平,分别较对照(照射0 min)显著降低87.85%、88.74%、91.91%、89.78%、93.04%和91.16%,且随处理时间延长其表达水平呈逐渐降低趋势。【结论】SfHsp70-1基因的过表达有助于白背飞虱对噻虫嗪和高温/低温胁迫的适应。研究结果可为进一步明确Hsp70基因在白背飞虱适应非生物胁迫中的作用奠定基础,并为制订白背飞虱绿色防控措施提供参考。[Objective]The role of heat shock protein Hsp70 gene in the adaptation of Sogatella furcifera to abiotic stresses(insecticide,high/low temperature,and UV-A)was revealed,which provided a reference for the green prevention and control of S.furcifera.[Method]Based on genomic and transcriptomic data,the Hsp70 gene in the S.furcifera was cloned and identified,and its transcription levels treated with insecti-cide,high/low temperature and UV-A stress were measured using RT-qPCR.[Result]A Hsp70 gene was successfully obtained and designat-ed as SfHsp70-1.Its fl-length sequence was determined to be 2322 bp,with an open reading frame of 2001 bp,encoding 666 amino acid res-idues,a molecular weight of approximately 72.91 kDa and a theoretical isoelectric point of 5.11.There were three conserved domains of the Hsp70 family proteins in SfHsp70-1,with a conserved C-terminal endoplasmic reticulum Hsp70 sequence‘HDEL'.The sublethal concentra-tion of thiamethoxam showed a significant dose effect,and LCio significantly induced the transcriptional level of the SfHsp70-1 gene to 2.59 fold that of the control(acetone treatment),whereas LC2s concentration stress showed no significant impact.Additionally,temperature stress at 10℃(30min),20℃(30,60min),and 40℃(10,30,60min)significantly induced thetranscriptional levelsof theSfHsp70-1gene by 77.24%,62.00%,85.89%,138.96%,75.67%and 61.10%compared to the control(25 C).UV-A(300μW/cm2)stress signifi-cantly inhibited the transcriptional levels of the SfHsp70-1 gene,with a reduction of 87.85%,88.74%,91.91%,89.78%,93.04%and 91.16%,respectively,compared to the control(0 min exposure),and the expression level showed a gradually downward trend with the ex-tension of treatment time.[Conclusion]The overexpression of the SfHsp70-I gene contributes to the S.furcifera adaptation to thiamethoxam and high/low temperature stress.These findings provide a foundation for further investigating the role of the Hsp70 gene in the adaptation of the S.furcifera to abiotic stress and providing a reference for

关 键 词：白背飞虱 热休克蛋白 HSP70基因 非生物胁迫 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]