HPLC-PDA联合Box-Behnken响应面法优选蜂胶片提取工艺  

HPLC-PDA Combined with Box-Behnken Response Surface Method to Optimize the Extraction Process of Propolis Tablets

作　　者：付恩桃 李会婷 王文宇 谷先锋 刘修树[1] FU Entao;LI Huiting;WANG Wenyu;GU Xianfeng;LIU Xiushu(Hefei Technology College,Hefei 230012,China;The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230036,China;Hefei Yaogu Health Technology Limited Company,Hefei 230011,China)

机构地区：[1]合肥职业技术学院,安徽合肥230012 [2]中国科学技术大学附属第一医院安徽省立医院,安徽合肥230036 [3]合肥耀谷健康科技有限公司,安徽合肥230011

出　　处：《长春师范大学学报》2024年第10期75-81,共7页Journal of Changchun Normal University

基　　金：安徽省高校自然科学研究重点项目“基于PI3K/AKT/mToR信号通路探讨瓜子金提取物改善AD小鼠认知功能的作用机制研究”(2023AH052545);安徽省药师协会“扶持计划”研究项目“吡仑帕奈血药浓度监测在癫痫患者特殊人群个体化给药中的应用研究”(SPSG2023001005);合肥职业技术学院横向科研项目“蜂胶总黄酮提取工艺及质量标准研究”(2023HXKT001);合肥职业技术学院科研重点项目“北沙参香豆素类提取及质量评价方法研究”(2024KJB06)。

摘　　要：建立HPLC-PDA联合Box-Behnken响应面法,优化蜂胶片的提取工艺。以提取时间、料液比、甲醇浓度为自变量,以白杨素和高良姜素含量的总评归一值为因变量,色谱柱为Hypersil ODS2 C18柱(4.6 mm×150 mm,5μm),流动相为甲醇-0.1%磷酸(58∶42),流速为1.0 mL/min;柱温为25℃,检测波长为268 nm;采用Box-Behnken响应面法优选蜂胶片的提取工艺,并进行验证。最佳提取工艺为30倍量83%甲醇提取,提取10 min。Box-Behnken响应面法优选的提取工艺稳定、可靠,可为蜂胶片的提取和质量控制提供参考。This study establishes HPLC-PDA combined with Box-Behnken response surface method to optimize the extraction process of propolis tablets.Extraction time,solid-liquid ratio and methanol concentration were independent variables,and the total normalized values of Chrysin and galangin contents were dependent variables.The chromatography was performed on Hypersil ODS2 C18 column(4.6 mm×150 mm,5μm),and the mobile phase consisted of methanol-0.1%phosphoric acid(58∶42)and the flow rate is 1.0 mL/min;The column temperature was 25℃and the detection wavelength was 268 nm.The Box-Behnken response surface method was used to optimize the extraction process of propolis tablets and verify it.The optimal extraction process was 30 times 83%methanol extraction and the extraction time was 10 min.The optimal extraction process of Box-Behnken response surface method is stable and reliable,which can provide reference for extraction and quality control of propolis tablets.

关 键 词：蜂胶片 Box-Behnken响应面法 白杨素 高良姜素 提取工艺 

分 类 号：R284.6[医药卫生—中药学]