硫化氢对渗透胁迫下酸枣种子萌发的影响  

Effect of Hydrogen Sulfide on the Germination of Jujube Seeds Under Osmotic Stress

作　　者：薛雯 李娜[1] 王朝平 樊修和 段禧龙 焦红红 于金高 XUE Wen;LI Na;WANG Chaoping;FAN Xiuhe;DUAN Xilong;JIAO Honghong;YU Jingao(Co-construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi&Education Ministry/State,Shaanxi Xianyang 712083,China;Key Laboratory of Research&Development of Characteristic Qin Medicine Resources(Cultivation),Shaanxi Xianyang 712083,China;Shaanxi University of Chinese Medicine/Shaanxi Innovative Drug Research Center,Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712083,China)

机构地区：[1]陕西中医药大学陕西中药资源产业化省部共建协同创新中心,陕西咸阳712046 [2]秦药特色资源研究开发国家重点实验室(培育),陕西咸阳712046 [3]陕西中医药大学陕西省创新药物研究中心,陕西咸阳712046

出　　处：《现代中医药》2024年第6期110-115,共6页Modern Chinese Medicine

基　　金：国家自然科学基金项目(82003964);陕西省科技厅重点研发计划社会发展领域一般项目(2024SF-YBXM-520);陕西省教育厅重点科学研究计划项目(23JY025);陕西省自然科学基金资助项目(2023-JC-QN-0996);陕西中医药大学2023年度研究生质量提升工程专项项目(CXSJ202334);陕西高校青年创新团队(2020)。

摘　　要：目的探究硫化氢(hydrogen sulfide,H_(2)S)对渗透胁迫下酸枣种子萌发的影响。方法用15%聚乙二醇6000(PEG-6000)模拟渗透胁迫,以硫氢化钠(sodium hydrosulfide,NaHS)作为外源H_(2)S供体,探究不同浓度(0.1、0.2、0.4、0.8、1.6 mM)NaHS溶液对酸枣种子萌发的促进作用。结果酸枣种子在渗透胁迫条件下其发芽受到显著抑制;在此基础上外源给予不同浓度NaHS溶液处理后,发芽指标(发芽率、发芽势、发芽指数、幼苗的胚根长、胚轴长)显著上升、氧化损伤程度[丙二醛(malonic dialdehyde,MDA)含量]显著下降,抗氧化能力[过氧化氢酶(catalase,CAT)活性]显著增强,渗透调节物质[可溶性糖(soluble sugars,SS)、脯氨酸(proline,Pro)]含量显著下降,其中0.4 mM NaHS溶液处理后效果最佳。结论一定浓度外源H_(2)S可通过增加CAT活性、调节SS和Pro的含量从而增强酸枣种子抗渗透胁迫的能力。本研究结果为H_(2)S参与酸枣抗逆性形成相关机制研究提供了一定参考。Objective To investigate the effect of hydrogen sulfide(H_(2)S)on the germination of jujube seeds under osmotic stress.Methods 15%polyethylene glycol 6000(PEG-6000)was used to simulate osmotic stress,and sodium hydrosulfide(NaHS)was used as an exogenous H_(2)S donor to investigate the promoting effect of different concentrations(0.1 mM,0.2 mM,0.4 mM,0.8 mM,1.6 mM)of NaHS solution on the germination of jujube seeds.Results The germination of jujube seeds was significantly inhibited under osmotic stress conditions;On this basis,exogenous treatment with different concentrations of NaHS solution resulted in a significant increase in germination indicators(germination rate,germination potential,germination index,seedling radicle length,hypocotyl length),a significant decrease in oxidative damage[malondialdehyde(MDA)content],a significant enhancement in antioxidant capacity[catalase(CAT)activity],and a significant decrease in osmoregulatory substances(soluble sugars,SS,proline,Pro)content.Among them,treatment with 0.4 mM NaHS solution showed the best effect.Conclusion A certain concentration of exogenous H_(2)S can enhance the ability of jujube seeds to resist osmotic stress by increasing CAT activity,regulating SS and Pro content.The results of this study provide some reference for the mechanism of H_(2)S involvement in the formation of stress resistance in jujube.

关 键 词：硫化氢 渗透胁迫 酸枣 种子萌发 渗透调节物质 

分 类 号：R282[医药卫生—中药学]