The Expression Characteristics and Potential Functions of Heat Shock Factors in Diatom Phaeodactylum tricornutum  

作　　者：Yanhuan Lin Jiaxin Feng Hao Fang Wei Huang Kanglie Guo Xiyan Liu Shuqi Wang Xiaojuan Liu 

机构地区：[1]Zhangzhou Institute of Technology,Zhangzhou,363000,China [2]Guangdong Provincial Key Laboratory of Marine Biotechnology and Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention,College of Sciences,Shantou University,Shantou,515063,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第10期2583-2596,共14页国际实验植物学杂志（英文）

基　　金：fundings of educational and scientific research projects for young and middle-aged teachers in Fujian Province(Grant number:2022JAT220693);Natural Science Foundation of Guangdong Province(Grant numbers:2024A1515013029 and 2022A1515012141).

摘　　要：Heat shock transcription factor(HSF)are essential regulators of heat shock protein(HSP)gene expression in plants and algae,contributing to their resilience against biotic and abiotic stresses.However,the localization,structure,phylogenetic relationship,and characteristics of PtHSF genes in microalgae,especially in diatom Phaeodactylum tricornutum,remain largely unexplored.This study presents a comprehensive analysis of the PtHSF gene family in P.tricornutum.A genome-wide analysis identified 68 PtHSF genes,which were classified into two distinct subfamilies:traditional and untraditional.Motif and structure analyses revealed evidence of multiple duplication events within the PtHSF gene family.Expression profiling revealed diurnal patterns,with 34 genes being downregulated during the light period and upregulated during the dark period,while 19 genes exhibited the opposite pattern.These findings suggest that PtHSF genes may have specialized functions during the diurnal cycle and play a crucial role in maintaining cellular homeostasis in response to various stresses.Notably,PtHSF16,30,and 43 genes exhibited higher expression levels,suggesting their potential importance.This study provides a valuable foundation for future investigations into the specific functions of HSFs under different stress conditions and their regulatory mechanisms in P.tricornutum and other microalgae.

关 键 词：Diurnal cycle heat shock factor DIATOM cellular homeostasis differentially expressed gene 

分 类 号：Q94[生物学—植物学]